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. 1981 Dec;68(6):1406–1408. doi: 10.1104/pp.68.6.1406

Metabolism of trans-Aconitic Acid in Maize 1

I. PURIFICATION OF TWO MOLECULAR FORMS OF CITRATE DEHYDRASE

David Brauer 1, Merle R Teel 1
PMCID: PMC426112  PMID: 16662117

Abstract

Trans-aconitate synthesis via citrate dehydrase was determined in crude extracts of maize (Zea mays L.) coleoptiles. Two molecular forms of this enzyme were purified by substrate-specific elution from DEAE-cellulose, ammonium sulfate precipitation, and gel filtration. Each molecular form migrates as a single band in isoelectric focusing. Gel filtration and sodium dodecyl sulfate electrophoresis provided evidence that one enzyme form is composed of four 80,000-dalton subunits while the other is composed of two 60,000-dalton subunits. There was no evidence of proteolytic conversion of the large to the small molecular weight form when the former was incubated with either the 15,000g supernatant or with proteases. The data indicate that the two molecular forms of citrate dehydrase are isozymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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