Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1982 Jan;69(1):67–71. doi: 10.1104/pp.69.1.67

Nucleotide Sequence Homology Exists between the Chloroplast and Nuclear Ribosomal DNAs of Euglena gracilis1

Stephanie E Curtis 1, James R Y Rawson 1
PMCID: PMC426147  PMID: 16662186

Abstract

The nuclear and chloroplast ribosomal DNAs from Euglena were shown to have specific regions of nucleotide sequence homology. The regions of homology were identified by hybridization of restriction endonuclease DNA fragments of cloned chloroplast and nuclear ribosomal DNAs to one another. The regions of homology between these two ribosomal DNAs were in that part of the genes that code for the 3′ end of the small rRNAs (16S and 19S) and near or at the DNA sequences coding for the 5S RNAs. The nucleotide sequence homology between these regions was estimated to be approximately 94% by the melting point depression of a hybrid formed between the two ribosomal DNAs.

Full text

PDF
67

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner T. I., Brenner D. J., Neufeld B. R., Britten R. J. Reduction in the rate of DNA reassociation by sequence divergence. J Mol Biol. 1973 Dec 5;81(2):123–135. doi: 10.1016/0022-2836(73)90184-8. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  3. Dyer T. A., Bowman C. M. Nucleotide sequences of chloroplast 5S ribosomal ribonucleic acid in flowering plants. Biochem J. 1979 Dec 1;183(3):595–604. doi: 10.1042/bj1830595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Getz M. J., Altenburg L. C., Saunders G. F. The use of RNA labeled in vitro with iodine-125 in molecular hybridization experiments. Biochim Biophys Acta. 1972 Dec 22;287(3):485–494. doi: 10.1016/0005-2787(72)90293-6. [DOI] [PubMed] [Google Scholar]
  5. Graf L., Kössel H., Stutz E. Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes. Nature. 1980 Aug 28;286(5776):908–910. doi: 10.1038/286908a0. [DOI] [PubMed] [Google Scholar]
  6. Gruol D., Rawson J. R., Haselkorn R. Ribosomal RNA genes in the nucleus and chloroplast of Euglena. Biochim Biophys Acta. 1975 Nov 18;414(1):20–29. doi: 10.1016/0005-2787(75)90122-7. [DOI] [PubMed] [Google Scholar]
  7. Heizmann P., Doly J., Hussein Y., Nicolas P., Nigon V., Bernardi G. The chloroplast genome of bleached mutants of Euglena gracilis. Biochim Biophys Acta. 1981 May 29;653(3):412–415. doi: 10.1016/0005-2787(81)90197-0. [DOI] [PubMed] [Google Scholar]
  8. Ingle J., Possingham J. V., Wells R., Leaver C. J., Loening U. E. The properties of chloroplast ribosomal-RNA. Symp Soc Exp Biol. 1970;24:303–325. [PubMed] [Google Scholar]
  9. Marco Y., Rochaix J. D. Organization of the nuclear ribosomal DNA of Chlamydomonas reinhardii. Mol Gen Genet. 1980;177(4):715–723. doi: 10.1007/BF00272684. [DOI] [PubMed] [Google Scholar]
  10. Orozco E. M., Jr, Gray P. W., Hallick R. B. Euglena gracilis chloroplast ribosomal RNA transcription units. I. The location of transfer RNA, 5 S, 16 S, and 23 S ribosomal RNA genes. J Biol Chem. 1980 Nov 25;255(22):10991–10996. [PubMed] [Google Scholar]
  11. Orozco E. M., Jr, Rushlow K. E., Dodd J. R., Hallick R. B. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions. J Biol Chem. 1980 Nov 25;255(22):10997–11003. [PubMed] [Google Scholar]
  12. Payne P. I., Corry M. J., Dyer T. A. Nucleotide sequence analysis of the cytoplasmic 5S ribosomal ribonucleic acid from five species of flowering plants. Biochem J. 1973 Dec;135(4):845–851. doi: 10.1042/bj1350845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rawson J. R., Boerma C. L. A measurement of the fraction of chloroplast DNA transcribed during chloroplast development in Euglena gracilis. Biochemistry. 1976 Feb 10;15(3):588–592. doi: 10.1021/bi00648a021. [DOI] [PubMed] [Google Scholar]
  14. Rawson J. R., Boerma C. L., Andrews W. H., Wilkerson C. G. Complexity and abundance of ribonucleic acid transcribed from restriction endonuclease fragments of Euglena chloroplast deoxyribonucleic acid during chloroplast development. Biochemistry. 1981 Apr 28;20(9):2639–2644. doi: 10.1021/bi00512a043. [DOI] [PubMed] [Google Scholar]
  15. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  16. Samols D. R., Hagenbuchle O., Gage L. P. Homology of the 3' terminal sequences of the 18S rRNA of Bombyx mori and the 16S rRNA of Escherchia coli. Nucleic Acids Res. 1979 Nov 10;7(5):1109–1119. doi: 10.1093/nar/7.5.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. Swanstrom R., Shank P. R. X-Ray Intensifying Screens Greatly Enhance the Detection by Autoradiography of the Radioactive Isotopes 32P and 125I. Anal Biochem. 1978 May;86(1):184–192. doi: 10.1016/0003-2697(78)90333-0. [DOI] [PubMed] [Google Scholar]
  19. Tewari K. K., Wildman S. G. Function of chloroplast DNA. I. Hybridization studies involving nuclear and chloroplast DNA with RNA from cytoplasmic (80S) and chloroplast (70S) ribosomes. Proc Natl Acad Sci U S A. 1968 Feb;59(2):569–576. doi: 10.1073/pnas.59.2.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Williams B. G., Blattner F. R. Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning. J Virol. 1979 Feb;29(2):555–575. doi: 10.1128/jvi.29.2.555-575.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES