Abstract
Cell wall preparations from green pericarp of normal and mutant Neverripe (Nr) and ripening inhibitor (rin) tomato (Lycopersicon esculentum Mill.) fruit were all equally degraded in vitro by a cell wall-bound protein extract from ripe normal tomatoes.
Similar cell wall-bound protein extracts from ripe Nr fruit were not as effective and those from ripe rin fruit gave no cell wall degradation at all in vitro. This was correlated with the absence of polygalacturonase in rin and low activity of Nr extracts.
Purified polygalacturonase was capable of in vitro cell wall degradation and it seems that this enzyme can account for the cell wall degradation observed with the total cell wall-bound protein extracts from ripe fruit.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DICKINSON D. B., MCCOLLUM J. P. CELLULASE IN TOMATO FRUITS. Nature. 1964 Aug 1;203:525–526. doi: 10.1038/203525a0. [DOI] [PubMed] [Google Scholar]
- Gross K. C., Wallner S. J. Degradation of Cell Wall Polysaccharides during Tomato Fruit Ripening. Plant Physiol. 1979 Jan;63(1):117–120. doi: 10.1104/pp.63.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hobson G. E. Polygalacturonase in normal and abnormal tomato fruit. Biochem J. 1964 Aug;92(2):324–332. doi: 10.1042/bj0920324. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Poovaiah B. W., Nukaya A. Polygalacturonase and Cellulase Enzymes in the Normal Rutgers and Mutant rin Tomato Fruits and Their Relationship to the Respiratory Climacteric. Plant Physiol. 1979 Oct;64(4):534–537. doi: 10.1104/pp.64.4.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tucker G. A., Robertson N. G., Grierson D. Changes in polygalacturonase isoenzymes during the 'ripening' of normal and mutant tomato fruit. Eur J Biochem. 1980 Nov;112(1):119–124. doi: 10.1111/j.1432-1033.1980.tb04993.x. [DOI] [PubMed] [Google Scholar]
- Wallner S. J., Bloom H. L. Characteristics of tomato cell wall degradation in vitro: implications for the study of fruit-softening enzymes. Plant Physiol. 1977 Aug;60(2):207–210. doi: 10.1104/pp.60.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wallner S. J., Walker J. E. Glycosidases in Cell Wall-degrading Extracts of Ripening Tomato Fruits. Plant Physiol. 1975 Jan;55(1):94–98. doi: 10.1104/pp.55.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]