Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 Jan;69(1):143–145. doi: 10.1104/pp.69.1.143

Carbamoyl Phosphate Synthetase Activity from the Cotyledons of Developing and Germinating Pea Seeds

Chris Kollöffel 1, Bep C Verkerk 1
PMCID: PMC426163  PMID: 16662147

Abstract

Carbamoyl phosphate synthetase activity was measured in partially purified extracts from cotyledons of developing and germinating seeds of Pisum sativum L. Some properties of the enzyme were established. During cotyledon development, the activity initially increased sharply but decreased during further development. The activity from germinating seeds was only one-tenth of the maximum activity at an early developmental phase. The results are discussed in relation to pea seed development and germination.

Full text

PDF
143

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bone D. H. Metabolism of Citrulline and Ornithine in Mung Bean Mitochondria. Plant Physiol. 1959 Mar;34(2):171–175. doi: 10.1104/pp.34.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kleczkowski K. L-Glutamine as donor of carbamoyl group nitrogen for the enzymic synthesis of citrulline in green pea seedlings. Acta Biochim Pol. 1965;12(3):243–249. [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. Levine R. L., Kretchmer N. Conversion of carbamoyl phosphate to hydroxyurea. An assay for carbamoylphosphate synthetase. Anal Biochem. 1971 Aug;42(2):324–337. doi: 10.1016/0003-2697(71)90044-3. [DOI] [PubMed] [Google Scholar]
  5. Lovatt C. J., Albert L. S. Regulation of Pyrimidine Biosynthesis in Intact Cells of Cucurbita pepo. Plant Physiol. 1979 Oct;64(4):562–569. doi: 10.1104/pp.64.4.562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Makoff A. J., Radford A. Genetics and biochemistry of carbamoyl phosphate biosynthesis and its utilization in the pyrimidine biosynthetic pathway. Microbiol Rev. 1978 Jun;42(2):307–328. doi: 10.1128/mr.42.2.307-328.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Murray D. R. Changes in Activities of Enzymes of Nitrogen Metabolism in Seedcoats and Cotyledons during Embryo Development in Pea Seeds. Plant Physiol. 1980 Oct;66(4):782–786. doi: 10.1104/pp.66.4.782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murray D. R. Nutritive Role of the Seedcoats during Embryo Development in Pisum sativum L. Plant Physiol. 1979 Nov;64(5):763–769. doi: 10.1104/pp.64.5.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Neal D., Naylor A. W. Purine and pyrimidine nucleotide inhibition of carbamyl phosphate synthetase from pea seedlings. Biochem Biophys Res Commun. 1968 May 10;31(3):322–327. doi: 10.1016/0006-291x(68)90478-6. [DOI] [PubMed] [Google Scholar]
  10. O'Neal T. D., Naylor A. W. Partial purification and properties of carbamoyl phosphate synthetase of Alaska pea (Pisum sativum L. cultivar Alaska). Biochem J. 1969 Jun;113(2):271–279. doi: 10.1042/bj1130271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'neal T. D., Naylor A. W. Some regulatory properties of pea leaf carbamoyl phosphate synthetase. Plant Physiol. 1976 Jan;57(1):23–28. doi: 10.1104/pp.57.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ong B. L., Jackson J. F. Pyrimidine nucleotide biosynthesis in Phaseolus aureus. Enzymic aspects of the control of carbamoyl phosphate synthesis and utilization. Biochem J. 1972 Sep;129(3):583–593. doi: 10.1042/bj1290583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ratner S. Enzymes of arginine and urea synthesis. Adv Enzymol Relat Areas Mol Biol. 1973;39:1–90. doi: 10.1002/9780470122846.ch1. [DOI] [PubMed] [Google Scholar]
  14. Shargool P. D., Steeves T., Weaver M., Russell M. The localization within plant cells of enzymes involved in arginine biosynthesis. Can J Biochem. 1978 Apr;56(4):273–279. doi: 10.1139/o78-042. [DOI] [PubMed] [Google Scholar]
  15. Storey R., Beevers L. Enzymology of Glutamine Metabolism Related to Senescence and Seed Development in the Pea (Pisum sativum L.). Plant Physiol. 1978 Apr;61(4):494–500. doi: 10.1104/pp.61.4.494. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES