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. 1982 Jan;69(1):214–219. doi: 10.1104/pp.69.1.214

Octopine Accumulation Early in Crown Gall Development is Progressive 1

Penelope Toothman 1,2
PMCID: PMC426176  PMID: 16662161

Abstract

A purification of octopine from crown gall tissue was developed to quantitate conversion of precursor [3H]arginine into [3H]octopine. Plant wound tissue which was sterile or infected with an avirulent strain of Agrobacterium tumefaciens did not accumulate detectable quantities of octopine, consistent with opine synthesis not being induced by wounding or infection. Octopine was only recovered from tissue infected with virulent tumor-inducing strains of A. tumefaciens. In every case tested, the morphological appearance of tumors preceded the accumulation of octopine by at least 1 week, and in some instances 3 weeks. Thus, what was necessary and sufficient for the expression of plant hormones (auxin and cytokinin) required for tumor growth was not sufficient for the accumulation of octopine. The possible nature of the temporal difference in the expression of hormone autotrophy and octopine synthesis is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAUN A. C. Conditioning of the host cell as a factor in the transformation process in crown gall. Growth. 1952 Jun;16(2):65–74. [PubMed] [Google Scholar]
  2. Braun A. C. A Physiological Basis for Autonomous Growth of the Crown-Gall Tumor Cell. Proc Natl Acad Sci U S A. 1958 Apr;44(4):344–349. doi: 10.1073/pnas.44.4.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chilton M. D., Drummond M. H., Merio D. J., Sciaky D., Montoya A. L., Gordon M. P., Nester E. W. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977 Jun;11(2):263–271. doi: 10.1016/0092-8674(77)90043-5. [DOI] [PubMed] [Google Scholar]
  4. Genetello C., Van Larebeke N., Holsters M., De Picker A., Van Montagu M., Schell J. Ti plasmids of Agrobacterium as conjugative plasmids. Nature. 1977 Feb 10;265(5594):561–563. doi: 10.1038/265561a0. [DOI] [PubMed] [Google Scholar]
  5. Grieshaber M. An enzymatic method for the extimation of octopine. Anal Biochem. 1976 Aug;74(2):600–603. doi: 10.1016/0003-2697(76)90244-x. [DOI] [PubMed] [Google Scholar]
  6. Grieshaber M., Kronig E., Koormann R. A photometric estmation of phospho-L-arginine, arginine and octopine using homogenous octopine dehydrogenase isoenzyme 2 from the squid, Loligo vulgaris Lam. Hoppe Seylers Z Physiol Chem. 1978 Jan;359(1):133–136. doi: 10.1515/bchm.1978.359.1.133. [DOI] [PubMed] [Google Scholar]
  7. Hack E., Kemp J. D. Purification and Characterization of the Crown Gall-specific Enzyme, Octopine Synthase. Plant Physiol. 1980 May;65(5):949–955. doi: 10.1104/pp.65.5.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kemp J. D. In Vivo Synthesis of Crown Gall-specific Agrobacterium tumefaciens-directed Derivatives of Basic Amino Acids. Plant Physiol. 1978 Jul;62(1):26–30. doi: 10.1104/pp.62.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kerr A., Manigault P., Tempé J. Transfer of virulence in vivo and in vitro in Agrobacterium. Nature. 1977 Feb 10;265(5594):560–561. doi: 10.1038/265560a0. [DOI] [PubMed] [Google Scholar]
  10. Klein R. M. RESISTANCE AND SUSCEPTIBILITY OF CARROT ROOTS TO CROWN-GALL TUMOR FORMATION. Proc Natl Acad Sci U S A. 1955 May 15;41(5):271–274. doi: 10.1073/pnas.41.5.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lippincott B. B., Lippincott J. A. Induction of responsiveness to octopine and lysopine in crown-gall tumors. Plant Physiol. 1975 Aug;56(2):213–215. doi: 10.1104/pp.56.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lippincott J. A., Lippincott B. B., Chang C. C. Promotion of crown-gall tumor growth by lysopine, octopine, nopaline, and carnosine. Plant Physiol. 1972 Feb;49(2):131–137. doi: 10.1104/pp.49.2.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MENAGE A., MOREL G. SUR LA PR'ESENCE D'OCTOPINE DANS LES TISSUS DE CROWN-GALL. C R Hebd Seances Acad Sci. 1964 Dec 21;259:4795–4796. [PubMed] [Google Scholar]
  14. ROCHE J., NGUYEN-VAN THOAI, ROBIN Y., GARCIA I., HATT J. L. Sur la nature et la répartition des guanidines monosubstituées dans les tissus des invertébrés; présence de dérivés metaboliques de l'arginine chez des mollusques, des crustacés et des echinodermes. C R Seances Soc Biol Fil. 1952 Dec;146(23-24):1899–1902. [PubMed] [Google Scholar]
  15. Reed R. G., MacKay C. M. Sensitive identification of the phenylthiohydantoin of arginine. Anal Biochem. 1978 Jan;84(1):324–327. doi: 10.1016/0003-2697(78)90517-1. [DOI] [PubMed] [Google Scholar]
  16. Robin Y., Guillou Y. An ion-exchange column chromatographic method for the isolation of octopine from Sepia officinalis L. muscle. Anal Biochem. 1977 Nov;83(1):45–51. doi: 10.1016/0003-2697(77)90507-3. [DOI] [PubMed] [Google Scholar]
  17. Rogler C. E. Plasmid-dependent temperature-sensitive phase in crown gall tumorigenesis. Proc Natl Acad Sci U S A. 1980 May;77(5):2688–2692. doi: 10.1073/pnas.77.5.2688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tempé J., Petit A., Holsters M., Montagu M., Schell J. Thermosensitive step associated with transfer of the Ti plasmid during conjugation: Possible relation to transformation in crown gall. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2848–2849. doi: 10.1073/pnas.74.7.2848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thomashow M. F., Nutter R., Montoya A. L., Gordon M. P., Nester E. W. Integration and organization of Ti plasmid sequences in crown gall tumors. Cell. 1980 Mar;19(3):729–739. doi: 10.1016/s0092-8674(80)80049-3. [DOI] [PubMed] [Google Scholar]
  20. Thomashow M. F., Nutter R., Postle K., Chilton M. D., Blattner F. R., Powell A., Gordon M. P., Nester E. W. Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6448–6452. doi: 10.1073/pnas.77.11.6448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Van Larebeke N., Genetello C., Schell J., Schilperoort R. A., Hermans A. K., Van Montagu M., Hernalsteens J. P. Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature. 1975 Jun 26;255(5511):742–743. doi: 10.1038/255742a0. [DOI] [PubMed] [Google Scholar]
  22. Watson B., Currier T. C., Gordon M. P., Chilton M. D., Nester E. W. Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol. 1975 Jul;123(1):255–264. doi: 10.1128/jb.123.1.255-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wiegman T., Woldring M. G., Pratt J. J. A new cocktail for liquid scintillation counting of aqueous radioimmunoassay-samples. Clin Chim Acta. 1975 Mar 24;59(3):347–356. doi: 10.1016/0009-8981(75)90010-8. [DOI] [PubMed] [Google Scholar]

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