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. 1982 Jan;69(1):234–239. doi: 10.1104/pp.69.1.234

Ribosomal RNA Synthesis in Soybean Suspension Cultures Growing in Different Media 1

Paul J Jackson 1,2, Karl G Lark 1
PMCID: PMC426180  PMID: 16662166

Abstract

The transcription of ribosomal RNA has been studied in suspension tissue cultures of soybean Glycine max L. Merr cv. Mandarin cells (SB-1). A large precursor molecule was synthesized which contains RNA homologous to the 25 and 18S cistrons. Transcription was from one strand and appeared to start adjacent to the 18S cistron and to proceed through the 18S DNA, a <0.6-kilobase transcribed spacer and the 25S cistron. A nontranscribed spacer region was identified. When cells grew rapidly in sucrose (24 hours doubling time) they contained 7 times as many ribosomes as when they grew slowly in maltose (200 hours doubling time). Upon transfer from maltose to sucrose, cells began to accumulate ribosomes at a rapid rate (80-fold more rapid synthesis than in maltose medium) within 2 hours at 33°C. The 2-hour lag is due in large part to a longer processing time during which newly synthesized RNA is packaged into ribosomes. Therefore, the increase in transcription rate may occur within a few minutes of the transfer to sucrose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dawid I. B., Wellauer P. K. A reinvestigation of 5' leads to 3' polarity in 40S ribosomal RNA precursor of Xenopus laevis. Cell. 1976 Jul;8(3):443–448. doi: 10.1016/0092-8674(76)90157-4. [DOI] [PubMed] [Google Scholar]
  2. KJELDGAARD N. O. The kinetics of ribonucleic acid- and protein formation in Salmonella typhimurium during the transition between different states of balance growth. Biochim Biophys Acta. 1961 Apr 29;49:64–76. doi: 10.1016/0006-3002(61)90870-8. [DOI] [PubMed] [Google Scholar]
  3. KURLAND C. G., MAALOE O. Regulation of ribosomal and transfer RNA synthesis. J Mol Biol. 1962 Mar;4:193–210. doi: 10.1016/s0022-2836(62)80051-5. [DOI] [PubMed] [Google Scholar]
  4. Leaver C. J., Key J. L. Ribosomal RNA synthesis in plants. J Mol Biol. 1970 May 14;49(3):671–680. doi: 10.1016/0022-2836(70)90290-1. [DOI] [PubMed] [Google Scholar]
  5. Limberg M., Cress D., Lark K. G. Variants of soybean cells which can grow in suspension with maltose as a carbon-energy source. Plant Physiol. 1979 Apr;63(4):718–721. doi: 10.1104/pp.63.4.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Reeder R. H., Higashinakagawa T., Miller O., Jr The 5' leads to 3' polarity of the Xenopus Ribosomal RNA precursor molecule. Cell. 1976 Jul;8(3):449–454. doi: 10.1016/0092-8674(76)90158-6. [DOI] [PubMed] [Google Scholar]
  7. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  8. Rogers M. E., Loening U. E., Fraser R. S. Ribosomal RNA precursors in plants. J Mol Biol. 1970 May 14;49(3):681–692. doi: 10.1016/0022-2836(70)90291-3. [DOI] [PubMed] [Google Scholar]
  9. Roman R. Replication of DNA by nuclei isolated from soybean suspension cultures. Plant Physiol. 1980 Oct;66(4):726–730. doi: 10.1104/pp.66.4.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  11. Verma D. P., Marcus A. Regulation of RNA synthesis in plant cell culture: delayed synthesis of ribosomal RNA during transition from the stationary phase to active growth. Dev Biol. 1973 Jan;30(1):104–114. doi: 10.1016/0012-1606(73)90050-x. [DOI] [PubMed] [Google Scholar]
  12. WADE H. E., MORGAN D. M. The nature of the fluctuating ribonucleic acid in Escherichia coli. Biochem J. 1957 Feb;65(2):321–331. doi: 10.1042/bj0650321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Waldron C., Lacroute F. Effect of growth rate on the amounts of ribosomal and transfer ribonucleic acids in yeast. J Bacteriol. 1975 Jun;122(3):855–865. doi: 10.1128/jb.122.3.855-865.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wu R., Kaiser A. D. Mapping the 5'-terminal nucleotides of the DNA of bacteriophage lambda and related phages. Proc Natl Acad Sci U S A. 1967 Jan;57(1):170–177. doi: 10.1073/pnas.57.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]

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