Abstract
The transport of l-arginine (l-Arg), l-aspartic acid (l-Asp), l-histidine (l-His), and l-phenylalanine (l-Phe) has been investigated in suspension cultures of Nicotiana tabacum cv. Wisconsin 38 cells. Uptake of these amino acids is pH- and energy-dependent, concentrative (except for l-Asp), stereospecific, dependent upon culture growth phase, and, apparently, carrier-mediated. A 1,000-fold higher concentration of l-leucine (l-Leu) can inhibit all of the energy-dependent uptake of l-Arg, l-Asp, l-His, and l-Phe. A 1,000-fold higher concentration of l-Arg or l-Phe can inhibit all of the energy-dependent uptake of l-Leu. l-Asp and l-His cannot inhibit all of the energy-dependent l-Leu uptake. However, evidence is presented which indicates that l-Leu enters the cell via the carrier system which also transports l-Asp and l-His. These inhibition data are consistent with the hypothesis that all of these amino acids are transported by a single carrier system.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berlin J., Mutert U. Evidence for distinct amino acid transport systems in cultured tobacco cells. Z Naturforsch C. 1978 Sep-Oct;33(9-10):641–645. doi: 10.1515/znc-1978-9-1006. [DOI] [PubMed] [Google Scholar]
- Blackman M. S., McDaniel C. N. Amino Acid Transport in Suspension-cultured Plant Cells: II. CHARACTERIZATION OF l-LEUCINE UPTAKE. Plant Physiol. 1980 Aug;66(2):261–266. doi: 10.1104/pp.66.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheruel J., Jullien M. Amino Acid Uptake into Cultivated Mesophyll Cells from Asparagus officinalis L. Plant Physiol. 1979 Apr;63(4):621–626. doi: 10.1104/pp.63.4.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheung Y. N., Nobel P. S. Amino Acid uptake by pea leaf fragments: specificity, energy sources, and mechanism. Plant Physiol. 1973 Dec;52(6):633–637. doi: 10.1104/pp.52.6.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harrington H. M., Smith I. K. Cysteine transport into cultured tobacco cells. Plant Physiol. 1977 Dec;60(6):807–811. doi: 10.1104/pp.60.6.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maretzki A., Thom M. Arginine and lysine transport in sugarcane cell suspension cultures. Biochemistry. 1970 Jun 23;9(13):2731–2736. doi: 10.1021/bi00815a022. [DOI] [PubMed] [Google Scholar]