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. 1982 Feb;69(2):296–299. doi: 10.1104/pp.69.2.296

Senescence and the Fluidity of Rose Petal Membranes 1

RELATIONSHIP TO PHOSPHOLIPID METABOLISM

Amihud Borochov 1,2, Abraham H Halevy 1,2, Meir Shinitzky 1,2
PMCID: PMC426197  PMID: 16662196

Abstract

In previous work, senescence of rose petal cells has been shown to be accompanied by a gradual decrease of membrane fluidity, as measured by a fluorescence polarization technique. Concomitantly, an increase in the free sterol-to-phospholipid ratio was found. Both observations were verified in this study. Further, experiments carried out on whole tissue and isolated protoplasts during senescence revealed that there was no quantitative change in the level of free sterols. The content of phospholipids decreased without any significant change in their composition. Results from experiments measuring the incorporation of [32P]orthophosphate indicated a reduced capacity for phospholipid synthesis in senescent cells. Both young and old tissue showed phospholipase A and D activity, the former increasing with age.

It was concluded that the fluidity of rose petal membranes decreases with age as a result of a decrease in phospholipid content, brought about by both reduced synthesis and enhanced degradation. Evidence supporting the view that the phenomena observed are related specifically to changes in the plasmalemma is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amar A., Rottem S., Razin S. Is the vertical disposition of Mycoplasma membrane proteins affected by membrane fluidity? Biochim Biophys Acta. 1979 Apr 19;552(3):457–467. doi: 10.1016/0005-2736(79)90190-1. [DOI] [PubMed] [Google Scholar]
  2. Beutelmann P., Kende H. Membrane Lipids in Senescing Flower Tissue of Ipomoea tricolor. Plant Physiol. 1977 May;59(5):888–893. doi: 10.1104/pp.59.5.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borochov A., Borochov H. Increase in membrane fluidity in liposomes and plant protoplasts upon osmotic swelling. Biochim Biophys Acta. 1979 Feb 2;550(3):546–549. doi: 10.1016/0005-2736(79)90156-1. [DOI] [PubMed] [Google Scholar]
  4. Borochov A., Halevy A. H. Microviscosity of plasmalemmas in rose petals as affected by age and environmental factors. Plant Physiol. 1978 May;61(5):812–815. doi: 10.1104/pp.61.5.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boss W. F., Ruesink A. W. Isolation and Characterization of Concanavalin A-labeled Plasma Membranes of Carrot Protoplasts. Plant Physiol. 1979 Dec;64(6):1005–1011. doi: 10.1104/pp.64.6.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bramhall S., Noack N., Wu M., Loewenberg J. R. A simple colorimetric method for determination of protein. Anal Biochem. 1969 Oct 1;31(1):146–148. doi: 10.1016/0003-2697(69)90251-6. [DOI] [PubMed] [Google Scholar]
  7. Cooper R. A., Arner E. C., Wiley J. S., Shattil S. J. Modification of red cell membrane structure by cholesterol-rich lipid dispersions. A model for the primary spur cell defect. J Clin Invest. 1975 Jan;55(1):115–126. doi: 10.1172/JCI107901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Demel R. A., De Kruyff B. The function of sterols in membranes. Biochim Biophys Acta. 1976 Oct 26;457(2):109–132. doi: 10.1016/0304-4157(76)90008-3. [DOI] [PubMed] [Google Scholar]
  9. Donaldson R. P., Beevers H. Lipid composition of organelles from germinating castor bean endosperm. Plant Physiol. 1977 Feb;59(2):259–263. doi: 10.1104/pp.59.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Galbraith D. W., Northcote D. H. The isolation of plasma membrane from protoplasts of soybean suspension cultures. J Cell Sci. 1977 Apr;24:295–310. doi: 10.1242/jcs.24.1.295. [DOI] [PubMed] [Google Scholar]
  11. Hodges T. K., Leonard R. T. Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods Enzymol. 1974;32:392–406. doi: 10.1016/0076-6879(74)32039-3. [DOI] [PubMed] [Google Scholar]
  12. Kagawa T., Lord J. M., Beevers H. The origin and turnover of organelle membranes in castor bean endosperm. Plant Physiol. 1973 Jan;51(1):61–65. doi: 10.1104/pp.51.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lord J. M., Kagawa T., Moore T. S., Beevers H. Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J Cell Biol. 1973 Jun;57(3):659–667. doi: 10.1083/jcb.57.3.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Macher B. A., Brown C. P., McManus T. T., Mudd J. B. Studies on Phospholipid-synthesizing Enzyme Activities during the Growth of Etiolated Cucumber Cotyledons. Plant Physiol. 1975 Jan;55(1):130–136. doi: 10.1104/pp.55.1.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mayak S., Vaadia Y., Dilley D. R. Regulation of Senescence in Carnation (Dianthus caryophyllus) by Ethylene: Mode of Action. Plant Physiol. 1977 Apr;59(4):591–593. doi: 10.1104/pp.59.4.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McKersie B. D., Thompson J. E. Phase Behavior of Chloroplast and Microsomal Membranes during Leaf Senescence. Plant Physiol. 1978 Apr;61(4):639–643. doi: 10.1104/pp.61.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rouser G., Fkeischer S., Yamamoto A. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids. 1970 May;5(5):494–496. doi: 10.1007/BF02531316. [DOI] [PubMed] [Google Scholar]
  18. Shiga T., Maeda N., Suda T., Kon K., Sekiya M. The decreased membrane fluidity of in vivo aged, human erythrocytes. A spin label study. Biochim Biophys Acta. 1979 May 3;553(1):84–95. doi: 10.1016/0005-2736(79)90032-4. [DOI] [PubMed] [Google Scholar]
  19. Shinitzky M., Barenholz Y. Fluidity parameters of lipid regions determined by fluorescence polarization. Biochim Biophys Acta. 1978 Dec 15;515(4):367–394. doi: 10.1016/0304-4157(78)90010-2. [DOI] [PubMed] [Google Scholar]
  20. Shinitzky M., Henkart P. Fluidity of cell membranes--current concepts and trends. Int Rev Cytol. 1979;60:121–147. [PubMed] [Google Scholar]
  21. Shinitzky M., Inbar M. Microviscosity parameters and protein mobility in biological membranes. Biochim Biophys Acta. 1976 Apr 16;433(1):133–149. doi: 10.1016/0005-2736(76)90183-8. [DOI] [PubMed] [Google Scholar]
  22. Suttle J. C., Kende H. Ethylene Action and Loss of Membrane Integrity during Petal Senescence in Tradescantia. Plant Physiol. 1980 Jun;65(6):1067–1072. doi: 10.1104/pp.65.6.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]

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