Figure 2.

Lipid hydrophobicity affects cell labeling efficiency of Anch, cA, and prehybridized strands. (a) Membrane incorporation compared to DAG for duplexes with 3′-cA₂₀-C16 strands and Anch strands bearing FA anchors of increasing length. (b) Membrane incorporation of 3′-cA₂₀-C16 and different Anch strands when added stepwise (white bars) or after preannealing (black bars). (c) Heatmap relating average membrane labeling (n = 3) to combinations of Anch and cA strand FA anchor lengths. Error bars are standard deviation of at least three independent measurements.