Abstract
Solid-state 15N- and [13C] NMR have been used to measure quantitatively the utilization of glycine in the presence of glutamine for the synthesis of storage protein in immature cotyledons of soybean (Glycine max L. cv. Elf) in culture. The presence of an equal molar amount of glycine in the medium causes a decrease in the use of glutamine-amide nitrogen. Glycine nitrogen is incorporated extensively into peptide bonds (in amounts greater than what would be expected if it appeared solely in glycine residues), but is used sparingly for synthesis of histidine ring residues, guanidino nitrogen residues of arginine, and lysine residues. The modest use of glycine carbon in protein synthesis does not parallel the use of glycine nitrogen.
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- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Schaefer J., Kier L. D., Stejskal E. O. Characterization of photorespiration in intact leaves using carbon dioxide labeling. Plant Physiol. 1980 Feb;65(2):254–259. doi: 10.1104/pp.65.2.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaefer J., Stejskal E. O., McKay R. A. Cross-polarization NMR of N-15 labeled soybeans. Biochem Biophys Res Commun. 1979 May 14;88(1):274–280. doi: 10.1016/0006-291x(79)91726-1. [DOI] [PubMed] [Google Scholar]
- Skokut T. A., Varner J. E., Schaefer J., Stejskal E. O., McKay R. A. [N]NMR determination of asparagine and glutamine nitrogen utilization for synthesis of storage protein in developing cotyledons of soybean in culture. Plant Physiol. 1982 Feb;69(2):308–313. doi: 10.1104/pp.69.2.308. [DOI] [PMC free article] [PubMed] [Google Scholar]