Abstract
Calcium phosphate-boric acid treatments and UDP-glucose both elicited aniline blue fluorescent, periodic acid-Schiff's reagent-resistant, deposits in association with the cell walls of cowpea (Vigna sinensis [Torner] Savi cv. Early Ramshorn) tissue. Those deposits induced by calcium phosphateboric acid treatment ultrastructurally resembled the “wound callose” commonly triggered by cell damage; they were formed in seemingly intact cells of stems and leaves and their formation was associated with an increase in the surface density of rough endoplasmic reticulum in the cell cytoplasm. In contrast, UDP-glucose induced a more rapid accumulation of aniline blue fluorescent material, but only at the cut edges of stem slices. Comparative light and electron microscopy indicated that the material was incorporated into the walls of the damaged cells, even when such cells were devoid of organized cytoplasm. These results indicate a difference in the mode and site of synthesis between wound callose and that elicited by exogenous UDP-glucose. They support the hypothesis that externally supplied UDP-glucose cannot be utilized by intact cells.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson R. L., Ray P. M. Labeling of the Plasma Membrane of Pea Cells by a Surface-localized Glucan Synthetase. Plant Physiol. 1978 May;61(5):723–730. doi: 10.1104/pp.61.5.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brett C. T. Synthesis of beta-(1-->3)-Glucan from Extracellular Uridine Diphosphate Glucose as a Wound Response in Suspension-cultured Soybean Cells. Plant Physiol. 1978 Sep;62(3):377–382. doi: 10.1104/pp.62.3.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAYOR H. D., HAMPTON J. C., ROSARIO B. A simple method for removing the resin from epoxy-embedded tissue. J Biophys Biochem Cytol. 1961 Apr;9:909–910. doi: 10.1083/jcb.9.4.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raymond Y., Fincher G. B., Maclachlan G. A. Tissue Slice and Particulate beta-Glucan Synthetase Activities from Pisum Epicotyls. Plant Physiol. 1978 Jun;61(6):938–942. doi: 10.1104/pp.61.6.938. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith M. M., McCully M. E. Enhancing aniline blue fluorescent staining of cell wall structures. Stain Technol. 1978 Mar;53(2):79–85. doi: 10.3109/10520297809111446. [DOI] [PubMed] [Google Scholar]
- Weibel E. R., Kistler G. S., Scherle W. F. Practical stereological methods for morphometric cytology. J Cell Biol. 1966 Jul;30(1):23–38. doi: 10.1083/jcb.30.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong Y. S., Maclachlan G. A. 1,3-beta-d-Glucanases from Pisum sativum Seedlings: III. DEVELOPMENT AND DISTRIBUTION OF ENDOGENOUS SUBSTRATES. Plant Physiol. 1980 Feb;65(2):222–228. doi: 10.1104/pp.65.2.222. [DOI] [PMC free article] [PubMed] [Google Scholar]