Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 Feb;69(2):514–521. doi: 10.1104/pp.69.2.514

Characteristics of Cultured Tomato Cells after Prolonged Exposure to Medium Containing Polyethylene Glycol 1

Avtar K Handa 1, Ray A Bressan 1, Sangita Handa 1, Paul M Hasegawa 1
PMCID: PMC426240  PMID: 16662239

Abstract

Cell lines of tomato (Lycopersicon esculentum Mill., cv. VFNT-Cherry) have been isolated, which are capable of growing in media containing polyethylene glycol (PEG) 6000 with water potentials as low as −15 or −22 bar. After prolonged exposure to media containing PEG, these cell populations have reduced abilities to grow in the absence of PEG. Upon resuspension in PEG-free medium, the cells swell and begin to release metabolites, including protein. Measurement by plasmometry of the osmotic potential of cells selected in medium with −22 bar water potential indicates that they maintain, at the end of the growth cycle, an osmotic potential of approximately −26 bar. This is compared to an osmotic potential of −9 bar for nonselected cells in medium without PEG, having an initial water potential of −4 bar. Thus, considerable osmotic adjustment occurs as a result of exposure to external low water potential. The results also indicate that PEG does not contribute significantly to osmotic adjustment of the cells.

Full text

PDF
514

Images in this article

516Image
on p.516

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amar L., Reinhold L. Loss of membrane transport ability in leaf cells and release of protein as a result of osmotic shock. Plant Physiol. 1973 Apr;51(4):620–625. doi: 10.1104/pp.51.4.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boos W. Bacterial transport. Annu Rev Biochem. 1974;43(0):123–146. doi: 10.1146/annurev.bi.43.070174.001011. [DOI] [PubMed] [Google Scholar]
  3. Delmer D. P. Dimethylsulfoxide as a potential tool for analysis of compartmentation in living plant cells. Plant Physiol. 1979 Oct;64(4):623–629. doi: 10.1104/pp.64.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Epstein E., Norlyn J. D., Rush D. W., Kingsbury R. W., Kelley D. B., Cunningham G. A., Wrona A. F. Saline culture of crops: a genetic approach. Science. 1980 Oct 24;210(4468):399–404. doi: 10.1126/science.210.4468.399. [DOI] [PubMed] [Google Scholar]
  5. Handa A. K., Bressan R. A. Assay of adenosine 3', 5' cyclic monophosphate by stimulation of protein kinase: a method not involving radioactivity. Anal Biochem. 1980 Mar 1;102(2):332–339. doi: 10.1016/0003-2697(80)90163-3. [DOI] [PubMed] [Google Scholar]
  6. Hanson A. D., Nelsen C. E. Betaine Accumulation and [C]Formate Metabolism in Water-stressed Barley Leaves. Plant Physiol. 1978 Aug;62(2):305–312. doi: 10.1104/pp.62.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Higinbotham N. Movement of ions and electrogenesis in higher plant cells. Am Zool. 1970 Aug;10(3):393–403. doi: 10.1093/icb/10.3.393. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Marx J. L. Plants: can they live in salt water and like it? Science. 1979 Dec 7;206(4423):1168–1169. doi: 10.1126/science.206.4423.1168. [DOI] [PubMed] [Google Scholar]
  10. Nieman R. H., Willis C. Correlation between the Suppression of Glucose and Phosphate Uptake and the Release of Protein from Viable Carrot Root Cells Treated with Monovalent Cations. Plant Physiol. 1971 Sep;48(3):287–293. doi: 10.1104/pp.48.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rubinstein B., Mahar P. Effects of Osmotic Shock on Some Membrane-regulated Events of Oat Coleoptile Cells. Plant Physiol. 1977 Mar;59(3):365–368. doi: 10.1104/pp.59.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schobert B. Is there an osmotic regulatory mechanism in algae and higher plants? J Theor Biol. 1977 Sep 7;68(1):17–26. doi: 10.1016/0022-5193(77)90224-7. [DOI] [PubMed] [Google Scholar]
  13. Zimmermann U., Steudle E. The pressure-dependence of the hydraulic conductivity, the membrane resistance and membrane potential during turgor pressure regulation in Valonia utricularis. J Membr Biol. 1974;16(4):331–352. doi: 10.1007/BF01872422. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES