Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 Feb;69(2):531–536. doi: 10.1104/pp.69.2.531

Alteration in the Acyl Lipid Composition of Thylakoids Induced by Aging and Its Effect on Thylakoid Structure 1

Landis E A Henry 1,2,3,2, Reto J Strasser 1,2,3, Paul-André Siegenthaler 1,2,3,3
PMCID: PMC426243  PMID: 16662242

Abstract

The alteration in both the lipid composition and chlorophyll proteins obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of detergent solubilized thylakoids was investigated on differentially aged spinach (Spinacia oleracea L.) chloroplasts. Freshly isolated material demonstrated seven major bands upon electrophoretic fractionation. Membranes aged in vitro showed a diminution and/or a disappearance of some bands concomitant with changes in the acyl lipid composition of these membranes. The extent of these changes was influenced by the purity of the preparation. Low temperature fluorescence measurements (77K) showed that upon aging, the photochemical capacity of photosystem II decreased prior to alterations in the molecular organization of the photochemical apparatus as indicated by the energy distribution between the two photosystems.

The results are discussed in terms of whether the series of changes in the acyl lipid composition upon aging is related to the variations in the electrophoretic pattern of the chlorophyll proteins.

Full text

PDF
531

Images in this article

534Image
on p.534
534Image
on p.534
535Image
on p.535

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Jackson C., Dench J., Moore A. L., Halliwell B., Foyer C. H., Hall D. O. Subcellular localisation and identification of superoxide dismutase in the leaves of higher plants. Eur J Biochem. 1978 Nov 15;91(2):339–344. doi: 10.1111/j.1432-1033.1978.tb12685.x. [DOI] [PubMed] [Google Scholar]
  3. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  4. Machold O., Meister A. Resolution of the light-harvesting chlorophyll a/b-protein of vicia faba chloroplasts into two different chlorophyll-protein complexes. Biochim Biophys Acta. 1979 Jun 5;546(3):472–480. doi: 10.1016/0005-2728(79)90082-3. [DOI] [PubMed] [Google Scholar]
  5. Nakatani H. Y., Barber J. An improved method for isolating chloroplasts retaining their outer membranes. Biochim Biophys Acta. 1977 Sep 14;461(3):500–512. [PubMed] [Google Scholar]
  6. Rawyler A., Siegenthaler P. A. Rapid analysis of membrane lipids using a combination of thin-layer chromatography and scanning of photographic negatives. J Biochem Biophys Methods. 1980 May;2(5):271–281. doi: 10.1016/0165-022x(80)90051-2. [DOI] [PubMed] [Google Scholar]
  7. Rawyler A., Siegenthaler P. A. Role of lipids in functions of photosynthetic membranes revealed by treatment with lipolytic acyl hydrolase. Eur J Biochem. 1980 Sep;110(1):179–187. doi: 10.1111/j.1432-1033.1980.tb04853.x. [DOI] [PubMed] [Google Scholar]
  8. Rich P. R., Boveris A., Bonner W. D., Jr, Moore A. L. Hydrogen peroxide generation by the alternate oxidase of higher plants. Biochem Biophys Res Commun. 1976 Aug 9;71(3):695–703. doi: 10.1016/0006-291x(76)90887-1. [DOI] [PubMed] [Google Scholar]
  9. Rocha V., Ting I. P. Malate dehydrogenases of leaf tissue from Spinacia oleracea: properties of three isoenzymes. Arch Biochem Biophys. 1971 Nov;147(1):114–122. doi: 10.1016/0003-9861(71)90316-x. [DOI] [PubMed] [Google Scholar]
  10. Shaw A. B., Anderson M. M., McCarty R. E. Role of Galactolipids in Spinach Chloroplast Lamellar Membranes: II. Effects of Galactolipid Depletion on Phosphorylation and Electron Flow. Plant Physiol. 1976 May;57(5):724–729. doi: 10.1104/pp.57.5.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Strasser R. J., Butler W. L. Energy transfer in the photochemical apparatus of flashed bean leaves. Biochim Biophys Acta. 1976 Dec 6;449(3):412–419. doi: 10.1016/0005-2728(76)90152-3. [DOI] [PubMed] [Google Scholar]
  12. Strasser R. J., Butler W. L. The yield of energy transfer and the spectral distribution of excitation energy in the photochemical apparatus of flashed bean leaves. Biochim Biophys Acta. 1977 Nov 17;462(2):295–306. doi: 10.1016/0005-2728(77)90128-1. [DOI] [PubMed] [Google Scholar]
  13. Tolbert N. E. Isolation of subcellular organelles of metabolism on isopycnic sucrose gradients. Methods Enzymol. 1974;31:734–746. doi: 10.1016/0076-6879(74)31077-4. [DOI] [PubMed] [Google Scholar]
  14. Wang C., Smith R. L. Lowry determination of protein in the presence of Triton X-100. Anal Biochem. 1975 Feb;63(2):414–417. doi: 10.1016/0003-2697(75)90363-2. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES