Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 Feb;69(2):546–548. doi: 10.1104/pp.69.2.546

Effects of Centrifugal Force and Centrifugation Time on the Sedimentation of Plant Organelles 1

Jerry Nagahashi 1, Kathleen Hiraike 1
PMCID: PMC426246  PMID: 16662245

Abstract

The effect of centrifugal force and length of centrifugation time on the sedimentation of plant organelles was determined for corn (Zea mays L.) root homogenates. A centrifugal force of 6000g for at least 20 minutes was necessary to pellet 90% of the mitochondrial marker (cytochrome c oxidase). This initial centrifugation step is optimal for separating mitochondria from microsomes, since cross-contamination of endoplasmic reticulum and plasma membrane vesicles with mitochondria is minimized. Centrifugal forces of 8000g or 10,000g for 20 minutes and 13,000g for 15 minutes pellet 90% of the mitochondrial marker; however, these centrifugation conditions also sediment more plasma membrane and endoplasmic reticulum.

Full text

PDF
546

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Day D. A., Wiskich J. T. The oxidation of malate and exogenous reduced nicotinamide adenine dinucleotide by isolated plant mitochondria. Plant Physiol. 1974 Jan;53(1):104–109. doi: 10.1104/pp.53.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hodges T. K., Leonard R. T. Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods Enzymol. 1974;32:392–406. doi: 10.1016/0076-6879(74)32039-3. [DOI] [PubMed] [Google Scholar]
  3. Ikuma H., Bonner W. D. Properties of Higher Plant Mitochondria. I. Isolation and Some Characteristics of Tightly-coupled Mitochondria from Dark-grown Mung Bean Hypocotyls. Plant Physiol. 1967 Jan;42(1):67–75. doi: 10.1104/pp.42.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Koehler D. E., Leonard R. T., Vanderwoude W. J., Linkins A. E., Lewis L. N. Association of latent cellulase activity with plasma membranes from kidney bean abscission zones. Plant Physiol. 1976 Sep;58(3):324–330. doi: 10.1104/pp.58.3.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Leonard R. T., Hanson J. B. Increased Membrane-bound Adenosine Triphosphatase Activity Accompanying Development of Enhanced Solute Uptake in Washed Corn Root Tissue. Plant Physiol. 1972 Mar;49(3):436–440. doi: 10.1104/pp.49.3.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leonard R. T., Hotchkiss C. W. Plasma Membrane-associated Adenosine Triphosphatase Activity of Isolated Cortex and Stele from Corn Roots. Plant Physiol. 1978 Feb;61(2):175–179. doi: 10.1104/pp.61.2.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leonard R. T., Vanderwoude W. J. Isolation of plasma membranes from corn roots by sucrose density gradient centrifugation: an anomalous effect of ficoll. Plant Physiol. 1976 Jan;57(1):105–114. doi: 10.1104/pp.57.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lilley R. M. Isolation of Functionally Intact Rhodoplasts from Griffithsia monilis (Ceramiaceae, Rhodophyta). Plant Physiol. 1981 Jan;67(1):5–8. doi: 10.1104/pp.67.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lord J. M., Kagawa T., Moore T. S., Beevers H. Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J Cell Biol. 1973 Jun;57(3):659–667. doi: 10.1083/jcb.57.3.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nagahashi J., Beevers L. Subcellular Localization of Glycosyl Transferases Involved in Glycoprotein Biosynthesis in the Cotyledons of Pisum sativum L. Plant Physiol. 1978 Mar;61(3):451–459. doi: 10.1104/pp.61.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sarkissian I. V., Srivastava H. K. On methods of isolation of active, tightly coupled mitochondria of wheat seedlings. Plant Physiol. 1968 Sep;43(9):1406–1410. doi: 10.1104/pp.43.9.1406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sullivan C. W., Volcani B. E. Isolation and characterization of plasma and smooth membranes of the marine diatom Nitzschia alba. Arch Biochem Biophys. 1974 Jul;163(1):29–45. doi: 10.1016/0003-9861(74)90451-2. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES