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. 1982 Mar;69(3):572–574. doi: 10.1104/pp.69.3.572

Isolation of a Wheat Cell Line with Altered Membrane Properties

László Erdei 1,2,3, László Vigh 1,2,3, Dénes Dudits 1,2,3
PMCID: PMC426256  PMID: 16662251

Abstract

A spontaneous dimethylsulfoxide (DMSO)-tolerant cell line was isolated from a cell culture of wheat (Triticum monococcum L.). The tolerant cells were able to grow in the presence of 4% DMSO. Cells formed from protoplasts of the tolerant line required DMSO for division in culture medium of high osmotic value.

Fatty acid composition and the molar ratio of phospholipids/sterols suggest a more ordered membrane structure in the tolerant line. Accordingly, a lower K+ influx rate was detected in the tolerant cells in comparison with the original line. These characteristics were maintained after 6 months' cultivation of the cells in DMSO-free growth medium. This suggested that genetic changes could be responsible for differences between the two cell lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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