Abstract
When [2,4-14C]porphobilinogen (PBG) or [2 (aminomethyl),5-14C]PBG is administered to etiolated barley (Hordeum vulgare L. var. Larker) leaves in darkness, label becomes incorporated into CO2, organic and amino acids, sugars, lipids, and proteins during a 4-hour incubation. Less than 1% of the label, however, is incorporated into porphyrins. The rate of 14CO2 evolution from leaves fed [2,4-14C]PBG is strongly inhibited by anaerobiosis but is unaffected by aminooxyacetic acid, while the rate of 14CO2 evolution from [2(aminomethyl),5-14C]PBG is strongly inhibited by aminooxyacetic acid but is not affected by anaerobiosis.
These results suggest that: (a) exogenous PBG is taken up and metabolized by etiolated barley leaves; (b) PBG is not metabolized exclusively to porphyrins but can be converted to a variety of intermediary metabolites; (c) this metabolism involves reactions which are partially dependent upon O2 and pyridoxal phosphate.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beale S. I., Castelfranco P. A. The Biosynthesis of delta-Aminolevulinic Acid in Higher Plants: II. Formation of C-delta-Aminolevulinic Acid from Labeled Precursors in Greening Plant Tissues. Plant Physiol. 1974 Feb;53(2):297–303. doi: 10.1104/pp.53.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARELL E. F., KAHN J. S. SYNTHESIS OF PORPHYRINS BY ISOLATED CHLOROPLASTS OF EUGLENA. Arch Biochem Biophys. 1964 Oct;108:1–6. doi: 10.1016/0003-9861(64)90347-9. [DOI] [PubMed] [Google Scholar]
- Duggan J. X., Meller E., Gassman M. L. Catabolism of 5-Aminolevulinic Acid to CO(2) by Etiolated Barley Leaves. Plant Physiol. 1982 Jan;69(1):19–22. doi: 10.1104/pp.69.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duggan J. X., Meller E., Gassman M. L. Catabolism of [1-C]levulinic Acid by etiolated and greening barley leaves. Plant Physiol. 1981 Oct;68(4):802–807. doi: 10.1104/pp.68.4.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gassman M. L. A Reversible Conversion of Phototransformable Protochlorophyll(ide)(656) to Photoinactive Protochlorophyll(ide)(656) by Hydrogen Sulfide in Etiolated Bean Leaves. Plant Physiol. 1973 Jan;51(1):139–145. doi: 10.1104/pp.51.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gurne D., Shemin D. The synthesis of porphobilinogen by immobilized delta-aminolevulinic acid dehydratase. Methods Enzymol. 1976;44:844–849. doi: 10.1016/s0076-6879(76)44061-2. [DOI] [PubMed] [Google Scholar]
- PERKINS H. J., ROBERTS D. W. Purification of chlorophylls, pheophytins and pheophorbides for specific activity determinations. Biochim Biophys Acta. 1962 Apr 23;58:486–498. doi: 10.1016/0006-3002(62)90059-8. [DOI] [PubMed] [Google Scholar]
- de la Roche A. I. Increase in linolenic Acid is not a prerequisite for development of freezing tolerance in wheat. Plant Physiol. 1979 Jan;63(1):5–8. doi: 10.1104/pp.63.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]