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. 1982 Apr;69(4):975–977. doi: 10.1104/pp.69.4.975

Demonstration of the Intercellular Compartmentation of l-Menthone Metabolism in Peppermint (Mentha piperita) Leaves 1

Rodney Croteau 1,2, Jerry N Winters 1
PMCID: PMC426338  PMID: 16662329

Abstract

The metabolism of l-menthone, which is synthesized in the epidermal oil glands of peppermint (Mentha piperita L. cv. Black Mitcham) leaves, is compartmented; on leaf maturity, this ketone is converted to l-menthol and l-menthyl acetate in one compartment, and to d-neomenthol and d-neomenthyl glucoside in a separate compartment. All of the enzymes involved in these reactions are soluble when prepared from whole-leaf homogenates. Mechanical separation of epidermal fragments from the mesophyll, followed by preparation of the soluble enzyme fraction from each tissue, revealed that the neomenthol dehydrogenase and the glucosyl transferase resided specifically in the mesophyll layer, whereas the menthol dehydrogenase and substantial amounts of the acetyl transferase were located in the epidermis, presumably within the epidermal oil glands. These results suggest that the compartmentation of menthone metabolism in peppermint leaves is intercellular, not intracellular.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv D., Krochmal E., Dantes A., Galun E. Biotransformation of monoterpenes by mentha cell lines: conversion of menthone to neomenthol. Planta Med. 1981 Jul;42(7):236–243. doi: 10.1055/s-2007-971633. [DOI] [PubMed] [Google Scholar]
  2. Burbott A. J., Loomis W. D. Effects of light and temperature on the monoterpenes of peppermint. Plant Physiol. 1967 Jan;42(1):20–28. doi: 10.1104/pp.42.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burbott A. J., Loomis W. D. Evidence for metabolic turnover of monoterpenes in peppermint. Plant Physiol. 1969 Feb;44(2):173–179. doi: 10.1104/pp.44.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Croteau R., Hooper C. L. Metabolism of Monoterpenes: Acetylation of (-)-Menthol by a Soluble Enzyme Preparation from Peppermint (Mentha piperita) Leaves. Plant Physiol. 1978 May;61(5):737–742. doi: 10.1104/pp.61.5.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Croteau R., Martinkus C. Metabolism of Monoterpenes: Demonstration of (+)-Neomenthyl-beta-d-Glucoside as a Major Metabolite of (-)-Menthone in Peppermint (Mentha Piperita). Plant Physiol. 1979 Aug;64(2):169–175. doi: 10.1104/pp.64.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Croteau R. Site of Monoterpene Biosynthesis in Majorana hortensis Leaves. Plant Physiol. 1977 Mar;59(3):519–520. doi: 10.1104/pp.59.3.519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Loomis W. D. Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 1974;31:528–544. doi: 10.1016/0076-6879(74)31057-9. [DOI] [PubMed] [Google Scholar]
  8. Martinkus C., Croteau R. Metabolism of Monoterpenes : EVIDENCE FOR COMPARTMENTATION OF l-MENTHONE METABOLISM IN PEPPERMINT (MENTHA PIPERITA) LEAVES. Plant Physiol. 1981 Jul;68(1):99–106. doi: 10.1104/pp.68.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]

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