Abstract
Three oxygenated unsaturated fatty acids were investigated to determine whether they were present in seedlings of corn (Zea mays L. cv. NK PX443) and sunflower (Helianthus annuus L. cv. Sundak). The three compounds, 13-hydroxy-12-oxo-cis-9-octadecenoic acid (I), 13-hydroxy-12-oxo-cis,cis-9, 15-octadecadienoic acid (II), and 12-oxo-cis,cis-10, 15-phy-todienoic acid (III), were detected and estimated by gas chromatography-mass spectrometry selected ion monitoring of their trimethylsilyloxy, methyloxime derivatives with 20-carbon analogs added as internal standards. In corn, the concentration of III increased between 5 and 10 days, while I and II remained relatively constant. A higher concentration of II was observed in corn seedlings grown in the light than those grown in the dark. Wounding increased the levels of all three compounds. In sunflower seedlings, the concentrations of I, II, and III increased between 6 and 10 days. The intracellular concentration of III in 10-day-old light-grown seedlings was estimated to be 200 nm in corn and 40 nm in sunflower.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Galliard T., Phillips D. R., Reynolds J. The formation of cis-3-nonenal, trans-2-nonenal and hexanal from linoleic acid hydroperoxide isomers by a hydroperoxide cleavage enzyme system in cucumber (Cucumis sativus) fruits. Biochim Biophys Acta. 1976 Aug 23;441(2):181–192. doi: 10.1016/0005-2760(76)90161-2. [DOI] [PubMed] [Google Scholar]
- Hara A., Radin N. S. Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem. 1978 Oct 1;90(1):420–426. doi: 10.1016/0003-2697(78)90046-5. [DOI] [PubMed] [Google Scholar]
- Vick B. A., Zimmerman D. C. Lipoxygenase and hydroperoxide lyase in germinating watermelon seedlings. Plant Physiol. 1976 May;57(5):780–788. doi: 10.1104/pp.57.5.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vick B. A., Zimmerman D. C. Lipoxygenase, hydroperoxide isomerase, and hydroperoxide cyclase in young cotton seedlings. Plant Physiol. 1981 Jan;67(1):92–97. doi: 10.1104/pp.67.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vick B. A., Zimmermann D. C. Distribution of a Fatty Acid cyclase enzyme system in plants. Plant Physiol. 1979 Aug;64(2):203–205. doi: 10.1104/pp.64.2.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmerman D. C., Vick B. A. Hydroperoxide isomerase: a new enzyme of lipid metabolism. Plant Physiol. 1970 Sep;46(3):445–453. doi: 10.1104/pp.46.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]