Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 May;69(5):1136–1139. doi: 10.1104/pp.69.5.1136

Effects of Glycine Hydroxamate, Carbon Dioxide, and Oxygen on Photorespiratory Carbon and Nitrogen Metabolism in Spinach Mesophyll Cells 1

Arthur L Lawyer 1,2,2, Karen L Cornwell 1,2, Sherry L Gee 1,2, James A Bassham 1,2
PMCID: PMC426372  PMID: 16662358

Abstract

The effects of added glycine hydroxamate on the photosynthetic incorporation of 14CO2 into metabolites by isolated mesophyll cells of spinach (Spinacia oleracea L.) was investigated under conditions favorable to photorespiratory (PR) metabolism (0.04% CO2 and 20% O2) and under conditions leading to nonphotorespiratory (NPR) metabolism (0.2% CO2 and 2.7% O2). Glycine hydroxamate (GH) is a competitive inhibitor of the photorespiratory conversion of glycine to serine, CO2 and NH4+. During PR fixation, addition of the inhibitor increased glycine and decreased glutamine labeling. In contrast, labeling of glycine decreased under NPR conditions. This suggests that when the rate of glycolate synthesis is slow, the primary route of glycine synthesis is through serine rather than from glycolate. GH addition increased serine labeling under PR conditions but not under NPR conditions. This increase in serine labeling at a time when glycine to serine conversion is partially blocked by the inhibitor may be due to serine accumulation via the “reverse” flow of photorespiration from 3-P-glycerate to hydroxypyruvate when glycine levels are high. GH increased glyoxylate and decreased glycolate labeling. These observations are discussed with respect to possible glyoxylate feedback inhibition of photorespiration.

Full text

PDF
1136

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Larsen P. O., Cornwell K. L., Gee S. L., Bassham J. A. Amino Acid Synthesis in Photosynthesizing Spinach Cells : EFFECTS OF AMMONIA ON POOL SIZES AND RATES OF LABELING FROM CO(2). Plant Physiol. 1981 Aug;68(2):292–299. doi: 10.1104/pp.68.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lawyer A. L., Berlyn M. B., Zelitch I. Isolation and Characterization of Glycine Hydroxamate-resistant Cell Lines of Nicotiana tabacum. Plant Physiol. 1980 Aug;66(2):334–341. doi: 10.1104/pp.66.2.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lawyer A. L., Cornwell K. L., Larsen P. O., Bassham J. A. Effects of carbon dioxide and oxygen on the regulation of photosynthetic carbon metabolism by ammonia in spinach mesophyll cells. Plant Physiol. 1981 Dec;68(6):1231–1236. doi: 10.1104/pp.68.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lawyer A. L., Zelitch I. Inhibition of glycine decarboxylation and serine formation in tobacco by glycine hydroxamate and its effect on photorespiratory carbon flow. Plant Physiol. 1979 Nov;64(5):706–711. doi: 10.1104/pp.64.5.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Oliver D. J., Zelitch I. Increasing photosynthesis by inhibiting photorespiration with glyoxylate. Science. 1977 Jun 24;196(4297):1450–1451. doi: 10.1126/science.867040. [DOI] [PubMed] [Google Scholar]
  6. Paul J. S., Bassham J. A. Maintenance of High Photosynthetic Rates in Mesophyll Cells Isolated from Papaver somniferum. Plant Physiol. 1977 Nov;60(5):775–778. doi: 10.1104/pp.60.5.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Platt S. G., Plaut Z., Bassham J. A. Steady-state photosynthesis in alfalfa leaflets: effects of carbon dioxide concentration. Plant Physiol. 1977 Aug;60(2):230–234. doi: 10.1104/pp.60.2.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Randall D. D., Tolbert N. E., Gremel D. 3-Phosphoglycerate Phosphatase in Plants: II. Distribution, Physiological Considerations, and Comparison with P-Glycolate Phosphatase. Plant Physiol. 1971 Oct;48(4):480–487. doi: 10.1104/pp.48.4.480. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES