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. 1982 Jun;69(6):1315–1319. doi: 10.1104/pp.69.6.1315

Vacuoles from Sugarcane Suspension Cultures 1

I. ISOLATION AND PARTIAL CHARACTERIZATION

Margaret Thom 1, Andrew Maretzki 1, Ewald Komor 1,2
PMCID: PMC426408  PMID: 16662393

Abstract

Vacuoles were isolated from suspension cultures of sugarcane (Saccharum sp.) cells by centrifugation of protoplasts at high g force against a 12% (w/v) Ficoll solution. Distribution of marker enzymes and Concanavalin A binding showed an 11% contamination of the vacuole preparation by cytoplasmic components, mitochondria, and endoplasmic reticulum, and 18% contamination by plasma membrane. Acid phosphatase, carboxypeptidase, protease, peroxidase, and ribonuclease activities were enriched in isolated vacuoles. Carboxypeptidase was tonoplast-bound, whereas the other enzymes were soluble. Sucrose, reducing sugars, and free amino acids were measured in protoplasts and vacuoles during growth of cells in suspension culture. Sucrose and reducing sugar content of vacuoles increased as the culture aged, while free amino acids decreased sharply.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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