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. 1982 Jun;69(6):1401–1403. doi: 10.1104/pp.69.6.1401

Uptake and Degradation of Cyclic AMP by Chloronema Cells

Shobhona Sharma 1, Man Mohan Johri 1
PMCID: PMC426426  PMID: 16662411

Abstract

Suspension cultures of intact chloronema cells of the moss Funaria hygrometrica take up [3H]cAMP and degrade it rapidly. The increase in total radioactivity accumulated by the cells was linear up to 30 minutes. Initially, the major degradation products were 5′-AMP and adenosine, but later predominantly ADP and ATP. In spite of rapid degradation, the amount of extracellularly applied cAMP retained by the cells is about 4-fold higher than the maximum endogenous level of cAMP reported previously (Handa, Johri 1977 Plant Physiol 59: 490-496). The uptake showed a distinct dependence on the density of the culture. Cells at a lower cell density (1-2 milligrams per milliliter) accumulated 4 to 6 times more radioactivity than the cells at high density (>10 milligrams per milliliter). The cyclic nucleotide phosphodiesterase (cNPDE) activity of whole cells (18 milliunits per milligram protein) was comparable to that of protoplasts (23 milliunits per milligram protein), but about 4-fold lower than that of lysed protoplasts (80 milliunits per milligram protein), indicating an intracellular degradation of cAMP by chloronema cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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