Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 Jun;69(6):1435–1438. doi: 10.1104/pp.69.6.1435

Alternative Pathway Respiration and Lipoxygenase Activity in Aged Potato Slice Mitochondria 1

Richard M Shingles 1,2, Geoffrey P Arron 1,3, Robert D Hill 1,4
PMCID: PMC426433  PMID: 16662418

Abstract

Mitochondrial preparations isolated from aged white potato (Solanum tuberosum L.) slices exhibited classical cyanide-insensitive O2 uptake which was inhibited by salicylhydroxamic acid and tetraethylthiuram disulfide (disulfiram). These mitochondria also possessed lipoxygenase activity, as determined by O2 uptake in the presence of 4 millimolar linoleic acid. Purification of the mitochondrial preparation on a continuous Percoll gradient resulted in a large decrease in lipoxygenase activity whereas cyanide-insensitive (disulfiram sensitive) O2 consumption was still observed. These data indicate that cyanide-insensitive O2 consumption in mitochondrial preparations isolated from aged white potato slices is of mitochondrial origin and not due to lipoxygenase contamination.

Full text

PDF
1435

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dizengremel P., Lance C. Control of Changes in Mitochondrial Activities during Aging of Potato Slices. Plant Physiol. 1976 Aug;58(2):147–151. doi: 10.1104/pp.58.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goldstein A. H., Anderson J. O., McDaniel R. G. Cyanide-insensitive and Cyanide-sensitive O(2) Uptake in Wheat: II. GRADIENT-PURIFIED MITOCHONDRIA LACK CYANIDE-INSENSITIVE RESPIRATION. Plant Physiol. 1981 Mar;67(3):594–596. doi: 10.1104/pp.67.3.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hackett D. P., Haas D. W., Griffiths S. K., Niederpruem D. J. Studies on Development of Cyanide-resistant Respiration in Potato Tuber Slices. Plant Physiol. 1960 Jan;35(1):8–19. doi: 10.1104/pp.35.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Henry M. F., Nyns E. D. Cyanide-insensitive respiration. An alternative mitochondrial pathway. Subcell Biochem. 1975 Mar;4(1):1–65. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Miller M. G., Obendorf R. L. Use of Tetraethylthiuram Disulfide to Discriminate between Alternative Respiration and Lipoxygenase. Plant Physiol. 1981 May;67(5):962–964. doi: 10.1104/pp.67.5.962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Parrish D. J., Leopold A. C. Confounding of alternate respiration by lipoxygenase activity. Plant Physiol. 1978 Sep;62(3):470–472. doi: 10.1104/pp.62.3.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schonbaum G. R., Bonner W. D., Jr, Storey B. T., Bahr J. T. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 1971 Jan;47(1):124–128. doi: 10.1104/pp.47.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Siedow J. N., Girvin M. E. Alternative Respiratory Pathway: ITS ROLE IN SEED RESPIRATION AND ITS INHIBITION BY PROPYL GALLATE. Plant Physiol. 1980 Apr;65(4):669–674. doi: 10.1104/pp.65.4.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Surrey K. Spectrophotometric Method for Determination of Lipoxidase Activity. Plant Physiol. 1964 Jan;39(1):65–70. doi: 10.1104/pp.39.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. THIMANN K. V., YOCUM C. S., HACKETT D. P. Terminal oxidases and growth in plant tissues. III. Terminal oxidation in potato tuber tissue. Arch Biochem Biophys. 1954 Nov;53(1):239–257. doi: 10.1016/0003-9861(54)90249-0. [DOI] [PubMed] [Google Scholar]
  12. Theologis A., Laties G. G. Membrane Lipid Breakdown in Relation to the Wound-induced and Cyanide-resistant Respiration in Tissue Slices: A COMPARATIVE STUDY. Plant Physiol. 1980 Nov;66(5):890–896. doi: 10.1104/pp.66.5.890. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES