Abstract
Low doses of rifampicin, an antibiotic that specifically inhibits RNA polymerase, cure F+ cells from the episome. An E. coli mutant, whose RNA polymerase is resistant to rifampicin, does not show the curing. The growth of Hfr strains, which replicate the chromosome by the F-replication system, is inhibited by low doses of the antibiotic.
Keywords: acridine orange, F+, F-, Hfr, lac
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishimura Y., Caro L., Berg C. M., Hirota Y. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J Mol Biol. 1971 Feb 14;55(3):441–456. doi: 10.1016/0022-2836(71)90328-7. [DOI] [PubMed] [Google Scholar]
- Tocchini-Valentini G. P., Marino P., Colvill A. J. Mutant of E. coli containing an altered DNA-dependent RNA polymerase. Nature. 1968 Oct 19;220(5164):275–276. doi: 10.1038/220275a0. [DOI] [PubMed] [Google Scholar]
- VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
- di Mauro E., Synder L., Marino P., Lamberti A., Coppo A., Tocchini-Valentini G. P. Rifampicin sensitivity of the components of DNA-dependent RNA polymerase. Nature. 1969 May 10;222(5193):533–537. doi: 10.1038/222533a0. [DOI] [PubMed] [Google Scholar]