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. 1995 Feb 14;92(4):1101–1104. doi: 10.1073/pnas.92.4.1101

Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris.

J Kami 1, V B Velásquez 1, D G Debouck 1, P Gepts 1
PMCID: PMC42645  PMID: 7862642

Abstract

Common bean (Phaseolus vulgaris) consists of two major geographic gene pools, one distributed in Mexico, Central America, and Colombia and the other in the southern Andes (southern Peru, Bolivia, and Argentina). Amplification and sequencing of members of the multigene family coding for phaseolin, the major seed storage protein of the common bean, provide evidence for accumulation of tandem direct repeats in both introns and exons during evolution of the multigene family in this species. The presumed ancestral phaseolin sequences, without tandem repeats, were found in recently discovered but nearly extinct wild common bean populations of Ecuador and northern Peru that are intermediate between the two major gene pools of the species based on geographical and molecular arguments. Our results illustrate the usefulness of tandem direct repeats in establishing the polarity of DNA sequence divergence and therefore in proposing phylogenies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anthony J. L., Vonder Haar R. A., Hall T. C. Nucleotide sequence of an alpha-phaseolin gene from Phaseolus vulgaris. Nucleic Acids Res. 1990 Jun 11;18(11):3396–3396. doi: 10.1093/nar/18.11.3396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Danner D. B. Recovery of DNA fragments from gels by transfer to DEAE-paper in an electrophoresis chamber. Anal Biochem. 1982 Sep 1;125(1):139–142. doi: 10.1016/0003-2697(82)90394-3. [DOI] [PubMed] [Google Scholar]
  3. Delwart E. L., Shpaer E. G., Louwagie J., McCutchan F. E., Grez M., Rübsamen-Waigmann H., Mullins J. I. Genetic relationships determined by a DNA heteroduplex mobility assay: analysis of HIV-1 env genes. Science. 1993 Nov 19;262(5137):1257–1261. doi: 10.1126/science.8235655. [DOI] [PubMed] [Google Scholar]
  4. Kami J. A., Gepts P. Phaseolin nucleotide sequence diversity in Phaseolus. I. Intraspecific diversity in Phaseolus vulgaris. Genome. 1994 Oct;37(5):751–757. doi: 10.1139/g94-107. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lerman L. S., Fischer S. G., Hurley I., Silverstein K., Lumelsky N. Sequence-determined DNA separations. Annu Rev Biophys Bioeng. 1984;13:399–423. doi: 10.1146/annurev.bb.13.060184.002151. [DOI] [PubMed] [Google Scholar]
  7. Ruano G., Kidd K. K. Modeling of heteroduplex formation during PCR from mixtures of DNA templates. PCR Methods Appl. 1992 Nov;2(2):112–116. doi: 10.1101/gr.2.2.112. [DOI] [PubMed] [Google Scholar]
  8. Slightom J. L., Drong R. F., Klassy R. C., Hoffman L. M. Nucleotide sequences from phaseolin cDNA clones: the major storage proteins from Phaseolus vulgaris are encoded by two unique gene families. Nucleic Acids Res. 1985 Sep 25;13(18):6483–6498. doi: 10.1093/nar/13.18.6483. [DOI] [PMC free article] [PubMed] [Google Scholar]

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