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. 1972 Feb;69(2):343–347. doi: 10.1073/pnas.69.2.343

On the Identity of DOPA Decarboxylase and 5-Hydroxytryptophan Decarboxylase

James G Christenson 1, Wallace Dairman 1, Sidney Udenfriend 1
PMCID: PMC426454  PMID: 4536745

Abstract

Simultaneous immunological titration of DOPA and 5-hydroxytryptophan decarboxylase activities, from a number of tissues of various species, showed that the two activities were not distinguishable with a monospecific antiserum to hog kidney decarboxylase. Together with previous findings, these data firmly establish the concept that in mammalian tissues the two enzyme activities are associated with a single protein, namely aromatic L-amino acid decarboxylase.

Keywords: immunological titration, aromatic L-amino acid decarboxylase, serotonin, dopamine, norepinephrine

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AWAPARA J., SANDMAN R. P., HANLY C. Activation of DOPA decarboxylase by pyridoxal phosphate. Arch Biochem Biophys. 1962 Sep;98:520–525. doi: 10.1016/0003-9861(62)90220-5. [DOI] [PubMed] [Google Scholar]
  2. BLASCHKO H., HAGEN P., WELCH A. D. Observations on the intracellular granules of the adrenal medulla. J Physiol. 1955 Jul 28;129(1):27–49. doi: 10.1113/jphysiol.1955.sp005336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartholini G., Da Prada M., Pletscher A. Decrease of cerebral 5-hydroxytryptamine by 3,4-dihydroxyphenylalanine after inhibition of extracerebral decarboxylase. J Pharm Pharmacol. 1968 Mar;20(3):228–229. doi: 10.1111/j.2042-7158.1968.tb09726.x. [DOI] [PubMed] [Google Scholar]
  4. Butcher L., Engel J., Fuxe K. L-dopa induced changes in central monoamine neurons after peripheral decarboxylase inhibition. J Pharm Pharmacol. 1970 Apr;22(4):313–316. doi: 10.1111/j.2042-7158.1970.tb08529.x. [DOI] [PubMed] [Google Scholar]
  5. CLARK C. T., WEISSBACH H., UDENFRIEND S. 5-Hydroxytryptophan decarboxylase: preparation and properties. J Biol Chem. 1954 Sep;210(1):139–148. [PubMed] [Google Scholar]
  6. Christenson J. G., Dairman W., Udenfriend S. Preparation and properties of a homogeneous aromatic L-amino acid decarboxylase from hog kidney. Arch Biochem Biophys. 1970 Nov;141(1):356–367. doi: 10.1016/0003-9861(70)90144-x. [DOI] [PubMed] [Google Scholar]
  7. Coulson W. F., Bender D. A., Jepson J. B. Multiple electrophoresis peaks of rat liver decarboxylases for 3,4-dihydroxyphenylalanine and 5-hydroxytryptophan. Biochem J. 1969 Dec;115(5):63P–64P. doi: 10.1042/bj1150063p. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FELLMAN J. H. Purification and properties of adrenal L-DOPA decarboxylase. Enzymologia. 1959 Jul 15;20:366–376. [PubMed] [Google Scholar]
  9. HAGEN P. Observations on the substrate specificity of DOPA decarboxylase from ox adrenal medulla, human phaeochromocytoma and human argentaffinoma. Br J Pharmacol Chemother. 1962 Feb;18:175–182. doi: 10.1111/j.1476-5381.1962.tb01161.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Henderson J. F., Brox L. W., Kelley W. N., Rosenbloom F. M., Seegmiller J. E. Kinetic studies of hypoxanthine-guanine phosphoribosyltransferase. J Biol Chem. 1968 May 25;243(10):2514–2522. [PubMed] [Google Scholar]
  11. LOVENBERG W., WEISSBACH H., UDENFRIEND S. Aromatic L-amino acid decarboxylase. J Biol Chem. 1962 Jan;237:89–93. [PubMed] [Google Scholar]
  12. Laduron P., Belpaire F. Tissue fractionation and catecholamines. II. Intracellular distribution patterns of tyrosine hydroxylase, dopa decarboxylase, dopamine-beta-hydroxylase, phenylethanolamine N-methyltransferase and monoamine oxidase in adrenal medulla. Biochem Pharmacol. 1968 Jul;17(7):1127–1140. doi: 10.1016/0006-2952(68)90048-8. [DOI] [PubMed] [Google Scholar]
  13. Lynch H. J. Diurnal oscillations in pineal melatonin content. Life Sci I. 1971 Jul 15;10(14):791–795. doi: 10.1016/0024-3205(71)90033-6. [DOI] [PubMed] [Google Scholar]
  14. Ng K. Y., Chase T. N., Colburn R. W., Kopin I. J. L-Dopa-induced release of cerebral monoamines. Science. 1970 Oct 2;170(3953):76–77. doi: 10.1126/science.170.3953.76. [DOI] [PubMed] [Google Scholar]
  15. ROSENGREN E. Are dihydroxphenylalanine decarboxylase and 5-hydroxytrptophan decarboxylase individual enzymes? Acta Physiol Scand. 1960 Aug 25;49:364–369. doi: 10.1111/j.1748-1716.1960.tb01958.x. [DOI] [PubMed] [Google Scholar]
  16. SNYDER S. H., ZWEIG M., AXELROD J., FISCHER J. E. CONTROL OF THE CIRCADIAN RHYTHM IN SEROTONIN CONTENT OF THE RAT PINEAL GLAND. Proc Natl Acad Sci U S A. 1965 Feb;53:301–305. doi: 10.1073/pnas.53.2.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Streffer C. Eine Methode zur Bestimmung der Dekarboxylase aromatischer Aminosäuren. Biochim Biophys Acta. 1967 May 16;139(1):193–195. doi: 10.1016/0005-2744(67)90132-5. [DOI] [PubMed] [Google Scholar]
  18. Udenfriend S. Biosynthesis of the sympathetic neurotransmitter, norepinephrine. Harvey Lect. 1966;60:57–83. [PubMed] [Google Scholar]
  19. WERLE E., AURES D. [On the purification and specificity of DOPA-decarboxylase]. Hoppe Seylers Z Physiol Chem. 1959 Sep 30;316:45–60. doi: 10.1515/bchm2.1959.316.1.45. [DOI] [PubMed] [Google Scholar]
  20. WESTERMANN E., BALZER H., KNELL J. Hemmung der Serotoninbildung durch alpha-Methyl-Dopa. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1958;234(3):194–205. [PubMed] [Google Scholar]
  21. Wurtman R. J., Axelrod J. A 24-hour rhythm in the content of norepinephrine in the pineal and salivary glands of the rat. Life Sci. 1966 Apr;5(7):665–669. doi: 10.1016/0024-3205(66)90298-0. [DOI] [PubMed] [Google Scholar]
  22. YUWILER A., GELLER E., EIDUSON S. Studies on 5-hydroxy-tryptophan decarboxylase. II. Additional inhibition studies and suggestions on the nature of the enzymic site. Arch Biochem Biophys. 1960 Jul;89:143–147. doi: 10.1016/0003-9861(60)90025-4. [DOI] [PubMed] [Google Scholar]

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