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. 1972 Feb;69(2):398–402. doi: 10.1073/pnas.69.2.398

Action of DNA Polymerase I of Escherichia coli with DNA-RNA Hybrids as Templates

John D Karkas 1, Jannis G Stavrianopoulos 1, Erwin Chargaff 1
PMCID: PMC426466  PMID: 4621833

Abstract

Experiments indicating the ability of the ribo strand of a DNA-RNA template to guide polydeoxynucleotide synthesis by highly purified DNA polymerase I of E. coli (EC 2.7.7.7) are presented. With poly(rA)·poly(dT) as template, poly(dT) is formed with a high efficiency, but almost no poly(dA). The specific activity of the enzyme, when tested with this template under suitable conditions, is eight times greater than that found for the poly(dA-dT) template. Single-stranded DNA fractions, with no template activity for DNA polymerase, are converted to efficient templates after their transcription by RNA polymerase. A concerted polymerization reaction, in which the action of DNA polymerase is dependent on that of RNA polymerase, can also be demonstrated with synthetic polydeoxynucleotides and single-stranded fractions of denatured DNA as templates.

Keywords: B. megaterium DNA, optimal conditions, high specific activity, synergistic RNA polymerase reaction

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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