Abstract
Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA polymerases are identified by their ability to copy efficiently the ribo strand of synthetic oligonucleotide-homopolymer complexes, and differ in this respect from the known DNA-dependent DNA polymerases found in HeLa cells. The template requirements of these new DNA polymerases resemble that of the RNA-dependent DNA polymerases of the RNA tumor-viruses.
Keywords: HeLa, fibroblast, synthetic oligonucleotides, template, purified enzymes
Full text
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
- Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berkowitz D. M., Kakefuda T., Sporn M. A simple and rapid method for the isolation of enzymatically active HeLa cell nuclei. J Cell Biol. 1969 Sep;42(3):851–854. doi: 10.1083/jcb.42.3.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bosmann H. B. RNA Directed DNA synthesis: Identification in L5178Y mouse leukemic cells and distribution of the polymerase in a synchronized L5178Y cell population. FEBS Lett. 1971 Feb 19;13(2):121–123. doi: 10.1016/0014-5793(71)80214-4. [DOI] [PubMed] [Google Scholar]
- Duesberg P., Helm K. V., Canaani E. Comparative properties of RNA and DNA templates for the DNA polymerase of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2505–2509. doi: 10.1073/pnas.68.10.2505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fridlender B., Weissbach A. DNA polymerases of tumor virus: specific effect of ethidium bromide on the use of different synthetic templates. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3116–3119. doi: 10.1073/pnas.68.12.3116. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gallo R. C., Yang S. S., Ting R. C. RNA dependent DNA polymerase of human acute leukaemic cells. Nature. 1970 Dec 5;228(5275):927–929. doi: 10.1038/228927a0. [DOI] [PubMed] [Google Scholar]
- Goodman N. C., Spiegelman S. Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203–2206. doi: 10.1073/pnas.68.9.2203. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayflick L., Stanbridge E. Isolation and identification of mycoplasma from human clinical materials. Ann N Y Acad Sci. 1967 Jul 28;143(1):608–621. doi: 10.1111/j.1749-6632.1967.tb27705.x. [DOI] [PubMed] [Google Scholar]
- Penner P. E., Cohen L. H., Loeb L. A. RNA-dependent DNA polymerase: presence in normal human cells. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1228–1234. doi: 10.1016/0006-291x(71)90037-4. [DOI] [PubMed] [Google Scholar]
- Schlabach A., Fridlender B., Bolden A., Weissbach A. DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization. Biochem Biophys Res Commun. 1971 Aug 20;44(4):879–885. doi: 10.1016/0006-291x(71)90793-5. [DOI] [PubMed] [Google Scholar]
- Scolnick E. M., Aaronson S. A., Todaro G. J., Parks W. P. RNA dependent DNA polymerase activity in mammalian cells. Nature. 1971 Jan 29;229(5283):318–321. doi: 10.1038/229318a0. [DOI] [PubMed] [Google Scholar]
- Stavrianopoulos J. G., Karkas J. D., Chargaff E. Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2207–2211. doi: 10.1073/pnas.68.9.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
- Todaro G. J., Aaronson S. A., Rands E. Rapid detection of mycoplasma-infected cell cultures. Exp Cell Res. 1971 Mar;65(1):256–257. doi: 10.1016/s0014-4827(71)80077-0. [DOI] [PubMed] [Google Scholar]
- Weissbach A., Schlabach A., Fridlender B., Bolden A. DNA polymerases from human cells. Nat New Biol. 1971 Jun 9;231(23):167–170. doi: 10.1038/newbio231167a0. [DOI] [PubMed] [Google Scholar]