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. 1972 Feb;69(2):452–455. doi: 10.1073/pnas.69.2.452

A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells

Bertold Fridlender 1,*, Michael Fry 1, Arthur Bolden 1, Arthur Weissbach 1
PMCID: PMC426478  PMID: 4501125

Abstract

Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA polymerases are identified by their ability to copy efficiently the ribo strand of synthetic oligonucleotide-homopolymer complexes, and differ in this respect from the known DNA-dependent DNA polymerases found in HeLa cells. The template requirements of these new DNA polymerases resemble that of the RNA-dependent DNA polymerases of the RNA tumor-viruses.

Keywords: HeLa, fibroblast, synthetic oligonucleotides, template, purified enzymes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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