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. 1972 Feb;69(2):480–484. doi: 10.1073/pnas.69.2.480

Direct Evidence for a Prostaglandin Receptor and Its Application to Prostaglandin Measurements

F A Kuehl Jr 1, J L Humes 1
PMCID: PMC426485  PMID: 4333988

Abstract

A prostaglandin receptor is present in rat adipocytes, as shown by competition studies with [3H]-prostaglandin E1. The affinity of the E prostaglandins for this receptor preparation is greater than that of A and F prostaglandins, an observation consistent with their relative potencies in stimulating cyclic AMP formation in isolated mouse ovaries and various other organs. In the protocol described, binding constants of 3-8 nM were obtained with prostaglandins E2 and E1. A sensitivity of 1 pmole makes this method readily applicable for measurement of tissue concentrations of the E prostaglandins.

Keywords: rat, adipocytes, antagonists, analogues, mouse ovary assay

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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