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. 1972 Feb;69(2):499–504. doi: 10.1073/pnas.69.2.499

Bacteriophage T7 DNA Replication: A Linear Replicating Intermediate*

John Wolfson 1, David Dressler 1, Marilyn Magazin 1
PMCID: PMC426489  PMID: 4551146

Abstract

The T7 chromosome in the first round of replication is a Y-shaped DNA rod. Thus, it differs from previously observed bacterial and viral replicating chromosomes that are circular.

Keywords: gradient centrifugation, electron microscopy, E. coli, DNA partial denaturation

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Selected References

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  1. Altman S., Lerman L. S. Effects of 9-aminoacridine on bacteriophage T4 deoxyribonucleic acid synthesis. J Mol Biol. 1970 Jun 14;50(2):263–277. doi: 10.1016/0022-2836(70)90191-9. [DOI] [PubMed] [Google Scholar]
  2. Bode H. R., Morowitz H. J. Size and structure of the Mycoplasma hominis H39 chromosome. J Mol Biol. 1967 Jan 28;23(2):191–199. doi: 10.1016/s0022-2836(67)80026-3. [DOI] [PubMed] [Google Scholar]
  3. Botstein D., Matz M. J. A recombination function essential to the growth of bacteriophage P22. J Mol Biol. 1970 Dec 28;54(3):417–440. doi: 10.1016/0022-2836(70)90119-1. [DOI] [PubMed] [Google Scholar]
  4. Carlson K. Intracellular fate of deoxyribonucleic acid from T7 bacteriophages. J Virol. 1968 Oct;2(10):1230–1233. doi: 10.1128/jvi.2.10.1230-1233.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Center M. S., Studier F. W., Richardson C. C. The structural gene for a T7 endonuclease essential for phage DNA synthesis. Proc Natl Acad Sci U S A. 1970 Jan;65(1):242–248. doi: 10.1073/pnas.65.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis R. W., Davidson N. Electron-microscopic visualization of deletion mutations. Proc Natl Acad Sci U S A. 1968 May;60(1):243–250. doi: 10.1073/pnas.60.1.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davis R. W., Hyman R. W. A study in evolution: the DNA base sequence homology between coliphages T7 and T3. J Mol Biol. 1971 Dec 14;62(2):287–301. doi: 10.1016/0022-2836(71)90428-1. [DOI] [PubMed] [Google Scholar]
  8. Dressler D. The rolling circle for phiX DNA replication. II. Synthesis of single-stranded circles. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1934–1942. doi: 10.1073/pnas.67.4.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dressler D., Wolfson J. The rolling circle for phi X DNA replication. 3. Synthesis of supercoiled duplex rings. Proc Natl Acad Sci U S A. 1970 Sep;67(1):456–463. doi: 10.1073/pnas.67.1.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frankel F. R. Evidence for long DNA strands in the replicating pool after T4 infection. Proc Natl Acad Sci U S A. 1968 Jan;59(1):131–138. doi: 10.1073/pnas.59.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  12. Green M., Piña M., Kimes R., Wensink P. C., MacHattie L. A., Thomas C. A., Jr Adenovirus DNA. I. Molecular weight and conformation. Proc Natl Acad Sci U S A. 1967 May;57(5):1302–1309. doi: 10.1073/pnas.57.5.1302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grippo P., Richardson C. C. Deoxyribonucleic acid polymerase of bacteriophage T7. J Biol Chem. 1971 Nov 25;246(22):6867–6873. [PubMed] [Google Scholar]
  14. Hausmann R., Gomez B. Amber mutants of bacteriophages T3 and T7 defective in phage-directed deoxyribonucleic acid synthesis. J Virol. 1967 Aug;1(4):779–792. doi: 10.1128/jvi.1.4.779-792.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hausmann R., Gomez B. Bacteriophage T3- and T7-directed deoxyribonucleases. J Virol. 1968 Mar;2(3):265–266. doi: 10.1128/jvi.2.3.265-266.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hirt B. Replicating molecules of polyoma virus DNA. J Mol Biol. 1969 Feb 28;40(1):141–144. doi: 10.1016/0022-2836(69)90302-7. [DOI] [PubMed] [Google Scholar]
  17. Huberman J. A., Riggs A. D. On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968 Mar 14;32(2):327–341. doi: 10.1016/0022-2836(68)90013-2. [DOI] [PubMed] [Google Scholar]
  18. Ihler G. M., Thomas C. A., Jr Equal incorporation of both parental bacteriophage T7 deoxyribonucleic acid strands into intracellular concatemeric deoxyribonucleic acid. J Virol. 1970 Dec;6(6):877–880. doi: 10.1128/jvi.6.6.877-880.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Inman R. B., Schnös M. Partial denaturation of thymine- and 5-bromouracil-containing lambda DNA in alkali. J Mol Biol. 1970 Apr 14;49(1):93–98. doi: 10.1016/0022-2836(70)90378-5. [DOI] [PubMed] [Google Scholar]
  20. Inselburg J., Fuke M. Replicating DNA: structure of colicin factor E1. Science. 1970 Aug 7;169(3945):590–592. doi: 10.1126/science.169.3945.590. [DOI] [PubMed] [Google Scholar]
  21. Kelly T. J., Jr, Thomas C. A., Jr An intermediate in the replication of bacteriophage T7 DNA molecules. J Mol Biol. 1969 Sep 28;44(3):459–475. doi: 10.1016/0022-2836(69)90373-8. [DOI] [PubMed] [Google Scholar]
  22. Kiger J. A., Jr, Sinsheimer R. L. DNA of vegetative bacteriophage lambda. VI. Electron microscopic studies of replicating lambda DNA. Proc Natl Acad Sci U S A. 1971 Jan;68(1):112–115. doi: 10.1073/pnas.68.1.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kirschner R. H., Wolstenholme D. R., Gross N. J. Replicating molecules of circular mitochondrial DNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1466–1472. doi: 10.1073/pnas.60.4.1466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Knippers R., Razin A., Davis R., Sinsheimer R. L. The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage. J Mol Biol. 1969 Oct 28;45(2):237–263. doi: 10.1016/0022-2836(69)90103-x. [DOI] [PubMed] [Google Scholar]
  25. Lee C. S., Davidson N. Physicochemical studies on the minicircular DNA in Escherichia coli 15. Biochim Biophys Acta. 1970 Apr 15;204(2):285–295. doi: 10.1016/0005-2787(70)90146-2. [DOI] [PubMed] [Google Scholar]
  26. Levine A. J., Kang H. S., Billheimer F. E. DNA replication in SV40 infected cells. I. Analysis of replicating SV40 DNA. J Mol Biol. 1970 Jun 14;50(2):549–568. doi: 10.1016/0022-2836(70)90211-1. [DOI] [PubMed] [Google Scholar]
  27. Masamune Y., Frenkel G. D., Richardson C. C. A mutant of bacteriophage T7 deficient in polynucleotide ligase. J Biol Chem. 1971 Nov 25;246(22):6874–6879. [PubMed] [Google Scholar]
  28. Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ogawa T., Tomizawa J. I., Fuke M. Replication of bacteriophage DNA, II. Structure of replicating DNA of phage lambda. Proc Natl Acad Sci U S A. 1968 Jul;60(3):861–865. doi: 10.1073/pnas.60.3.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ohki M., Tomizawa J. Asymmetric transfer of DNA strands in bacterial conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:651–658. doi: 10.1101/sqb.1968.033.01.074. [DOI] [PubMed] [Google Scholar]
  31. Ray D. S. Replication of bacteriophage M13. II. The role of replicative forms in single-strand synthesis. J Mol Biol. 1969 Aug 14;43(3):631–643. doi: 10.1016/0022-2836(69)90364-7. [DOI] [PubMed] [Google Scholar]
  32. Ritchie D. A., Thomas C. A., Jr, MacHattie L. A., Wensink P. C. Terminal repetition in non-permuted T3 and T7 bacteriophage DNA molecules. J Mol Biol. 1967 Feb 14;23(3):365–376. doi: 10.1016/s0022-2836(67)80111-6. [DOI] [PubMed] [Google Scholar]
  33. Rupp W. D., Ihler G. Strand selection during bacterial mating. Cold Spring Harb Symp Quant Biol. 1968;33:647–650. doi: 10.1101/sqb.1968.033.01.073. [DOI] [PubMed] [Google Scholar]
  34. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  35. Schnös M., Inman R. B. Position of branch points in replicating lambda DNA. J Mol Biol. 1970 Jul 14;51(1):61–73. doi: 10.1016/0022-2836(70)90270-6. [DOI] [PubMed] [Google Scholar]
  36. Schnös M., Inman R. B. Starting point and direction of replication in P2 DNA. J Mol Biol. 1971 Jan 14;55(1):31–38. doi: 10.1016/0022-2836(71)90278-6. [DOI] [PubMed] [Google Scholar]
  37. Smith M. G., Skalka A. Some properties of DNA from phage-infected bacteria. J Gen Physiol. 1966 Jul;49(6):127–142. doi: 10.1085/jgp.49.6.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Studier F. W., Hausmann R. Integration of two sets of T7 mutants. Virology. 1969 Nov;39(3):587–588. doi: 10.1016/0042-6822(69)90106-8. [DOI] [PubMed] [Google Scholar]
  39. Studier F. W., Maizel J. V., Jr T7-directed protein synthesis. Virology. 1969 Nov;39(3):575–586. doi: 10.1016/0042-6822(69)90105-6. [DOI] [PubMed] [Google Scholar]
  40. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  41. WATSON J. D., CRICK F. H. The structure of DNA. Cold Spring Harb Symp Quant Biol. 1953;18:123–131. doi: 10.1101/sqb.1953.018.01.020. [DOI] [PubMed] [Google Scholar]
  42. Werner R. Distribution of growing points in DNa of bacteriophage T4. J Mol Biol. 1968 May 14;33(3):679–692. doi: 10.1016/0022-2836(68)90313-6. [DOI] [PubMed] [Google Scholar]
  43. Westmoreland B. C., Szybalski W., Ris H. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy. Science. 1969 Mar 21;163(3873):1343–1348. doi: 10.1126/science.163.3873.1343. [DOI] [PubMed] [Google Scholar]

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