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. 1972 Feb;69(2):510–514. doi: 10.1073/pnas.69.2.510

Assignment of Three Human Genes to Chrosomes (LDH-A to 11, TK to 17, and IDH to 20) and Evidence for Translocation between Human and Mouse Chrosomes in Somatic Cell Hybrids

Charlotte Boone 1, Tchaw-Ren Chen 1, Frank H Ruddle 1
PMCID: PMC426491  PMID: 4110482

Abstract

Independently derived man-mouse somatic cell hybrids and their derivative subclones show a positive correlation between the expression of human lactate dehydrogenase A subunits and the occurrence of the human C-11 chromosome. Data are also presented that confirm the previously reported linkage of the thymidine kinase locus to the E-17 chromosome. A translocation of the E-17 chromosome provides presumptive evidence for the assignment of the thymidine kinase locus to the long arm segment of the E-17 chromosome. This translocation also provides evidence for translocation between man and mouse chromosomes in somatic cell hybrids. A presumptive association between the human phenotype for isocitrate dehydrogenase and the human F group is also described. Identification of specific human chromosomes was achieved by the application of several new cytological techniques: measurement of chromosome arm length, in situ annealing with mouse satellite complementary RNA, constitutive heterochromatin staining with Giemsa, and quinacrine mustard fluorochromatic staining.

Keywords: thymidine kinase; lactate dehydrogenase A; isocitrate dehydrogenase; C-11, E-17, and F-20 chromosomes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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