Table 1.

Antibody titre against horse serum IgG, mixed with mannosamine–biotin

	First injection antibody titre		Second injection antibody titre
Immunizing antigen	Mean	SD	Mean	SD
hsIgG	753	217	15 237	2254
hsIgG + MBA (not conjugated)	659	260	14 600	3010
hsIgG + BSA–MBA	720	310	13 100	3940
hsIgG–MBA (conjugated)	< 32a		77a	15

Mice were immunized twice, at 2-week intervals, with horse IgG (hsIgG), hsIgG mixed with mannosamine–biotin (MBA) or haptenated hsIgG. Anti-carrier (hsIgG) antibody titre was determined as the reciprocal value of the twofold serial dilution end-point (twice the negative control OD value) of the serum as tested by ELISA (n = 4 to 6). Titres < 32 are equal to non-immune serum.

^aP < 0.05 for the differences between anti-carrier antibody titre levels following immunization with MBA-conjugated carrier and other treatments.