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. 1972 Feb;69(2):520–522. doi: 10.1073/pnas.69.2.520

Inhibition of Release of Dopamine-β-Hydroxylase and Norepinephrine from Sympathetic Nerves by Colchicine, Vinblastine, or Cytochalasin-B

Nguyen B Thoa 1,*, G Frederick Wooten 1, Julius Axelrod 1, Irwin J Kopin 1
PMCID: PMC426493  PMID: 4333989

Abstract

Stimulation of the hypogastric nerve to the guinea pig vas deferens in the presence of phenoxybenzamine produced an enhanced release of both dopamine-β-hydroxylase (EC 1.14.2.1) and norepinephrine. Addition of colchicine, vinblastine, or cytochalasin-B to the incubation medium caused an almost complete inhibition of release of both the amine and the enzyme. Colchicine and cytochalasin-B produced a blocking effect but did not, however, modify the ratio of dopamine-β-hydroxylase to norepinephrine recovered in the bath fluid. The findings indicate that norepinephrine and dopamine-β-hydroxylase are released from sympathetic nerve terminals by exocytosis and that this process is dependent upon the integrity of both microtubules and microfilaments.

Keywords: hypogastric nerve stimulation, exocytosis, microtubules, microfilaments, guinea pig

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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