Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Mar;69(3):533–534. doi: 10.1073/pnas.69.3.533

Human Placental 15-Hydroxyprostaglandin Dehydrogenase

Joseph Jarabak 1
PMCID: PMC426500  PMID: 4501572

Abstract

Normal, term, human placentas are a rich source of a 15-hydroxyprostaglandin dehydrogenase. The enzyme is extremely labile, and partial purification could be achieved only after stabilization with glycerol. The instability of the enzyme and its Km for NAD are indications that it is different from the 15-hydroxyprostaglandin dehydrogenase isolated from swine lung. Human placental tissue should provide a very useful source from which large amounts of highly purified 15-hydroxyprostaglandin dehydrogenase may be obtained.

Keywords: glycerol stabilization, pyridine nucleotide-linked enzyme, enzyme kinetics

Full text

PDF
533

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anggård E., Larsson C., Samuelsson B. The distribution of 15-hydroxy prostaglandin dehydrogenase and prostaglandin-delta 13-reductase in tissues of the swine. Acta Physiol Scand. 1971 Mar;81(3):396–404. doi: 10.1111/j.1748-1716.1971.tb04914.x. [DOI] [PubMed] [Google Scholar]
  2. Anggård E. The biological activities of three metabolites of prostaglandin E 1. Acta Physiol Scand. 1966 Apr;66(4):509–510. doi: 10.1111/j.1748-1716.1966.tb03231.x. [DOI] [PubMed] [Google Scholar]
  3. Ferreira S. H., Vane J. R. Prostaglandins: their disappearance from and release into the circulation. Nature. 1967 Dec 2;216(5118):868–873. doi: 10.1038/216868a0. [DOI] [PubMed] [Google Scholar]
  4. Granström E., Samuelsson B. On the metabolism of prostaglandin F-2-alpha in female subjects. J Biol Chem. 1971 Sep 10;246(17):5254–5263. [PubMed] [Google Scholar]
  5. Karim S. M., Devlin J. Prostaglandin content of amniotic fluid during pregnancy and labour. J Obstet Gynaecol Br Commonw. 1967 Apr;74(2):230–234. doi: 10.1111/j.1471-0528.1967.tb14866.x. [DOI] [PubMed] [Google Scholar]
  6. Karim S. M. The role of prostaglandins in human parturition. Proc R Soc Med. 1971 Jan;64(1):10–12. [PMC free article] [PubMed] [Google Scholar]
  7. Nakano J., Anggård E., Samuelsson B. 15-Hydroxy-prostanoate dehydrogenase. Prostaglandins as substrates and inhibitors. Eur J Biochem. 1969 Dec;11(2):386–389. doi: 10.1111/j.1432-1033.1969.tb00784.x. [DOI] [PubMed] [Google Scholar]
  8. Nakano J., Monatague B., Darrow B. Metabolism of prostaglandin E1 in human plasma, uterus and placenta, in swine ovary and in rat testicle. Biochem Pharmacol. 1971 Sep;20(9):2512–2514. doi: 10.1016/0006-2952(71)90254-1. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES