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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Mar;69(3):585–589. doi: 10.1073/pnas.69.3.585

Increased Uptake of Amino Acids and 2-Deoxy-D-Glucose by Virus-Transformed Cells in Culture

K J Isselbacher 1,*
PMCID: PMC426512  PMID: 4335068

Abstract

Transformed and nontransformed cells in tissue culture differ in their rate of uptake of certain nutrients, as determined by a polyester-coverslip technique. A 2.5- to 3.5-fold increased rate of uptake of α-aminoisobutyric acid, cycloleucine, and 2-deoxy-D-glucose was observed with polyoma virus-transformed baby hamster kidney (BHK) 21 cells and simian virus 40 (SV40)-transformed BALB/3T3 (mouse fibroblast) cells, compared to their nontransformed counterparts. Kinetic analysis suggested that the increased uptake by cells transformed with virus was associated with a 3-fold greater Vmax, with no detectable changes in apparent Km. Limited studies also revealed increased initial rates of uptake by murine sarcoma virus-transformed rat liver cells, as compared to the parental line. Exposure of cells to concanavalin A and wheat-germ agglutinin led to significant reductions in amino-acid uptake by both transformed and nontransformed cells; however, transformed cells showed a greater decrease in uptake after exposure to wheat-germ agglutinin. Increased initial rates of uptake of certain amino acids and sugars may be a feature common to transformed cells, compared to their parental control.

Keywords: lectins, mouse, hamster, SV40, polyoma

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Selected References

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