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. 1972 Mar;69(3):676–680. doi: 10.1073/pnas.69.3.676

A Mutant of Escherichia coli That Requires High Concentrations of Biotin

Allan Campbell 1, Alice del Campillo-Campbell 1, Robin Chang 1
PMCID: PMC426533  PMID: 4551983

Abstract

A recessive mutation (bir) of E. coli, at 77 min on the standard map, destroys ability of the bacteria to take up biotin or to grow except at high concentrations of biotin. In a biotin prototroph, the bir mutation causes overproduction and excretion of biotin, and derepression of at least one biosynthetic gene (bioD). Growth of the bir mutant is inhibited by avidin or high concentrations of dethiobiotin. Both effects are reversed by biotin.

Keywords: transport, repression, binding sites, dethiobiotin

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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