Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Mar;69(3):690–693. doi: 10.1073/pnas.69.3.690

Bioluminescence: Mechanism and Mode of Control of Scintillon Activity

Margaret Fogel 1,*, J W Hastings 1,
PMCID: PMC426536  PMID: 4501583

Abstract

Subcellular particles, termed scintillons, isolated from the luminescent dinoflagellate Gonyaulax, emit light when the pH is lowered from 8 to 5.7. The capacity to emit a second flash is regained by incubation of scintillons with the low molecular weight luciferin from Gonyaulax. This compound was previously demonstrated to be the substrate in the bioluminescent reaction catalyzed by a soluble luciferase isolated from the same cell. A model of the scintillon is presented that involves soluble elements structured within a membrane-bound particle.

Keywords: dinoflagellates, Gonyaulax, luciferase, control mechanisms

Full text

PDF
690

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BODE V. C., HASTINGS J. W. THE PURIFICATION AND PROPERTIES OF THE BIOLUMINESCENT SYSTEM IN GONYAULAX POLYEDRA. Arch Biochem Biophys. 1963 Dec;103:488–499. doi: 10.1016/0003-9861(63)90442-9. [DOI] [PubMed] [Google Scholar]
  2. DeSa R., Hastings J. W. The characterization of scintillons. Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra. J Gen Physiol. 1968 Jan;51(1):105–122. doi: 10.1085/jgp.51.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eckert R., Reynolds G. T. The subcellular origin of bioluminescence in Noctiluca miliaris. J Gen Physiol. 1967 May;50(5):1429–1458. doi: 10.1085/jgp.50.5.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fogel M., Hastings J. W. A substrate-binding protein in the Gonyaulax bioluminescence reaction. Arch Biochem Biophys. 1971 Jan;142(1):310–321. doi: 10.1016/0003-9861(71)90289-x. [DOI] [PubMed] [Google Scholar]
  5. Hastings J. W., Mitchell G., Mattingly P. H., Blinks J. R., Van Leeuwen M. Response of aequorin bioluminescence to rapid changes in calcium concentration. Nature. 1969 Jun 14;222(5198):1047–1050. doi: 10.1038/2221047a0. [DOI] [PubMed] [Google Scholar]
  6. Krieger N., Hastings J. W. Bioluminescence: pH activity profiles of related luciferase fractions. Science. 1968 Aug 9;161(3841):586–589. doi: 10.1126/science.161.3841.586. [DOI] [PubMed] [Google Scholar]
  7. Schmitter R. E. The fine structure of Gonyaulax polyedra, a bioluminescent marine dinoflagellate. J Cell Sci. 1971 Jul;9(1):147–173. doi: 10.1242/jcs.9.1.147. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES