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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Mar;69(3):761–764. doi: 10.1073/pnas.69.3.761

Lac Repressor Binding to Synthetic DNAs of Defined Nucleotide Sequence

A D Riggs 1, S Lin 1, R D Wells *
PMCID: PMC426552  PMID: 4501591

Abstract

Binding of lac repressor to 20 synthetic DNAs of high molecular weight with defined repeating sequences was investigated by competition experiments. Although none of these DNAs binds repressor as tightly as does lac operator, most do bind to a measurable extent. Their affinity for repressor varies greatly and is a function of both nucleotide composition and sequence. Poly(dC-dC)·poly(dG-dC) competes for repressor 200-times less well than either poly(dA-dT)·poly(dA-dT) or poly(dT-dT-dG)·poly(dC-dA-dA). The other DNAs show a broad spectrum of affinities for repressor between these extremes. These results show that the lac repressor has affinity for, and can distinguish between, sequences distantly related to its operator.

Keywords: nearest-neighbor analysis, rate competition experiments, equilibrium dissociation constants

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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