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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Parasitology. 2014 Jun 16;141(14):1841–1855. doi: 10.1017/S0031182014000626

Fig. 4.

Fig. 4

Performance of the UCP-CCA assay. In the UCP-CCA assay the anti-CCA antibody #54-4C2 is immobilized at 200 ng per 4 mm on the T line of the LF strip (capture) and covalently coupled to the UCP reporter particle 25 μg per mg UCP reporter (detection). (Panel A) Ratio values determined with the UCP-CCA assay; analysis is of an AWA-TCA (3% w/w CCA) standard series in buffer, urine and serum. (Panel B) The UCP-CCA ratio value of 29 urine reference samples (various origins) compared with the visually recorded semi-quantitative POC-CCA test. The POC-CCA (RMD) scores are semi-quantified as: 0, trace, 1, 2, 3 or 4; 1 indicates the assay threshold below which samples are classified as no response (0) or a trace signal (TS), 1–4 indicate a low, medium, medium-high and high response, respectively.