Table 1.
Description and assay conditions |
UCP-LF ratioa |
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Samplesb | Format | Batchc | Referenced | Average | Stdev | Max | Low | High |
the Netherlands 97 | Dry | 2010xy | SCORE 2010_1 | 0·013 | 0·0078 | 0·033 | 0·029 | 0·049 |
Burundi 86 | Dry | 2010xy | SCORE 2010_1 | 0·019 | 0·0076 | 0·036 | 0·034 | 0·051 |
Senegal 130 | Dry | 2010xy | SCORE 2010_1 | 0·028 | 0·0080 | 0·050 | 0·044 | 0·066 |
South Africa 86 | Dry | 2009pq | van Dam et al. (2013) | 0·023 | 0·0075 | 0·050 | 0·038 | 0·065 |
the Netherlands 30 | Wet | 2008zz | Corstjens et al. (2008) | 0·037 | 0·0087 | 0·064 | 0·053 | 0·081 |
Average, average ratio of the negatives; Stdev, S.D.; Max, largest ratio value determined in the selection; Low and High represent low- and high-specificity cut-off threshold. In contrast to the high specificity threshold, the low specificity threshold may not lead to 100% clinical specificity. Results obtained utilizing the 20 μL assay for CAA detection in serum (SCAA20).
the Netherlands, Burundi, Senegal: sera were also used in previous studies developing the CAA- and CCA-ELISA (Krijger et al. 1994; Polman et al. 2000). South Africa: as described in Van Dam et al. (2013). the Netherlands, as described in Corstjens et al. (2008). Digits indicate the number of sera tested.
Different batches of LF strips and UCP conjugate may influence the UCP-LF ratio value. Batches are identified by production year and two letters indicating the LF strip and UCP batch, respectively.
Results from Burundi, the Netherlands and Senegal group for the UCP-CAA LF assay (unpublished) were reported to SCORE for the 2010 annual progress report.