Table 1.

Variation in the UCP-LF CAA cut-off threshold when testing different populations

Description and assay conditions				UCP-LF ratioa
Samplesb	Format	Batchc	Referenced	Average	Stdev	Max	Low	High
the Netherlands 97	Dry	2010xy	SCORE 2010_1	0·013	0·0078	0·033	0·029	0·049
Burundi 86	Dry	2010xy	SCORE 2010_1	0·019	0·0076	0·036	0·034	0·051
Senegal 130	Dry	2010xy	SCORE 2010_1	0·028	0·0080	0·050	0·044	0·066
South Africa 86	Dry	2009pq	van Dam et al. (2013)	0·023	0·0075	0·050	0·038	0·065
the Netherlands 30	Wet	2008zz	Corstjens et al. (2008)	0·037	0·0087	0·064	0·053	0·081

^aAverage, average ratio of the negatives; Stdev, S.D.; Max, largest ratio value determined in the selection; Low and High represent low- and high-specificity cut-off threshold. In contrast to the high specificity threshold, the low specificity threshold may not lead to 100% clinical specificity. Results obtained utilizing the 20 μL assay for CAA detection in serum (SCAA20).

^bthe Netherlands, Burundi, Senegal: sera were also used in previous studies developing the CAA- and CCA-ELISA (Krijger et al. 1994; Polman et al. 2000). South Africa: as described in Van Dam et al. (2013). the Netherlands, as described in Corstjens et al. (2008). Digits indicate the number of sera tested.

^cDifferent batches of LF strips and UCP conjugate may influence the UCP-LF ratio value. Batches are identified by production year and two letters indicating the LF strip and UCP batch, respectively.

^dResults from Burundi, the Netherlands and Senegal group for the UCP-CAA LF assay (unpublished) were reported to SCORE for the 2010 annual progress report.