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. 1972 Apr;69(4):824–828. doi: 10.1073/pnas.69.4.824

Aryl Hydrocarbon Hydroxylase and Polycyclic Hydrocarbon Tumorigenesis: Effect of the Enzyme Inhibitor 7,8-Benzoflavone on Tumorigenesis and Macromolecule Binding

Nadao Kinoshita 1, Harry V Gelboin 1
PMCID: PMC426573  PMID: 4502935

Abstract

Aryl hydrocarbon hydroxylase is present and is inducible in mouse skin. 7,8-Benzoflavone, an inhibitor of the enzyme, markedly inhibits tumorigenesis by 7,12-dimethylbenz(a)anthracene, but has either no effect on or stimulates benzo(a)pyrene tumorigenesis. Thus, the role of aryl hydrocarbon hydroxylase appears highly specific for each polycyclic hydrocarbon, in respect to detoxification and/or activation of the hydrocarbon to a carcinogenic form. In parallel studies, we found that 7,8-benzoflavone significantly reduces the amount of 7,12-dimethylbenz(a)anthracene binding to mouse skin DNA, RNA, and protein, and the binding of benzo(a)pyrene to RNA and protein of mouse skin. 7,8-Benzoflavone exhibited a markedly lesser effect on the binding of benzo(a)pyrene to DNA.

Keywords: benzo(a)pyrene; 7,12-dimethylbenz(a)anthracene; DNA, RNA, and protein binding; mouse skin

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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