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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Apr;69(4):1003–1007. doi: 10.1073/pnas.69.4.1003

Effects of Palmitoyl CoA on Citrate and Malate Transport by Rat Liver Mitochondria

M L Halperin 1,2,3, B H Robinson 1,2,3, I B Fritz 1,2,3
PMCID: PMC426613  PMID: 4502928

Abstract

Palmitoyl CoA inhibited citrate transport from isolated rat liver mitochondria. Under conditions described, 50% inhibition was observed at about 6-8 nmol/mg of mitochondrial protein per ml. The percentage inhibition was inversely proportional to the concentration of the counter-transporting anion in the medium. Although comparable levels of palmitoyl CoA had little effect on malate exit on the dicarboxylate carrier, higher concentrations inhibited both citrate and malate transport.

The specificity of the inhibition by palmitoyl CoA was investigated by examination of the effects on the tricarboxylate transport system of fatty acids, CoASH, acetyl CoA, palmitoylcarnitine, deoxycholate, and other molecules with surface active properties. None of the above compounds, at sublytic concentrations, inhibited citrate transport appreciably. The inhibition of citrate transport by low concentrations of palmitoyl CoA was rapid and could be prevented or partially reversed by addition of albumin.

Keywords: tricarboxylate transport system, fatty acid synthesis, surface molecules, metabolic regulation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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