Abstract
Sedimentation analysis of nuclear DNA released from spheroplasts of the yeast Saccharomyces cerevisiae indicates that it has a number average molecular weight of 6.2 × 108. The chromosomal DNA molecules range in size from as small as 5 × 107 daltons to as large as 1.4 × 109 daltons. Based on these values and estimates of the total DNA content of the yeast nucleus, it is proposed that each yeast chromosome contains a single DNA duplex.
Keywords: eukaryote, high molecular weight DNA, unineme, spheroplast
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- BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUELL E. A., INOUE S., UTTER M. F. ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST. J Bacteriol. 1964 Dec;88:1762–1773. doi: 10.1128/jb.88.6.1762-1773.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freifelder D. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. J Mol Biol. 1970 Dec 28;54(3):567–577. doi: 10.1016/0022-2836(70)90127-0. [DOI] [PubMed] [Google Scholar]
- Gray H. B., Jr, Hearst J. E. Flexibility of native DNA from the sedimentation behavior as a function of molecular weight and temperature. J Mol Biol. 1968 Jul 14;35(1):111–129. doi: 10.1016/s0022-2836(68)80041-5. [DOI] [PubMed] [Google Scholar]
- Grossman L. I., Goldring E. S., Marmur J. Preferential synthesis of yeast mitochondrial DNA in the absence of protein synthesis. J Mol Biol. 1969 Dec 28;46(3):367–376. doi: 10.1016/0022-2836(69)90182-x. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H. Biochemical genetics of yeast. Annu Rev Genet. 1970;4:373–396. doi: 10.1146/annurev.ge.04.120170.002105. [DOI] [PubMed] [Google Scholar]
- Hollenberg C. P., Borst P., van Bruggen E. F. Mitochondrial DNA. V. A 25 micron closed circular duplex DNA molecule in wild-type yeast mitochondria. Stucture and genetic complexity. Biochim Biophys Acta. 1970 May 21;209(1):1–15. [PubMed] [Google Scholar]
- LEUCHTENBERGER C., LEUCHTENBERGER R., DAVIS A. M. A microspectrophotometric study of the desoxyribose nucleic acid (DNA) content in cells of normal and malignant human tissues. Am J Pathol. 1954 Jan-Feb;30(1):65–85. [PMC free article] [PubMed] [Google Scholar]
- Lehmann A. R., Ormerod M. G. Double-strand breaks in the DNA of a mammalian cell after x-irradiation. Biochim Biophys Acta. 1970 Oct 15;217(2):268–277. doi: 10.1016/0005-2787(70)90526-5. [DOI] [PubMed] [Google Scholar]
- Leighton S. B., Rubenstein I. Calibration of molecular weight scales for DNA. J Mol Biol. 1969 Dec 14;46(2):313–328. doi: 10.1016/0022-2836(69)90424-0. [DOI] [PubMed] [Google Scholar]
- van der Vliet P. C., Tonino G. J., Rozijn T. H. Studies on the yeast nucleus. 3. Properties of a deoxyribonucleoprotein complex derived from yeast. Biochim Biophys Acta. 1969 Dec 16;195(2):473–483. [PubMed] [Google Scholar]