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. 1972 May;69(5):1249–1252. doi: 10.1073/pnas.69.5.1249

Interaction of Eukaryote Elongation Factor EF 1 with Guanosine Nucleotides and Aminoacyl-tRNA

Hong-Mo Moon 1, Betty Redfield 1, Herbert Weissbach 1
PMCID: PMC426674  PMID: 4556458

Abstract

Evidence for two species of elongation factor 1 (EF 1A and EF 1B) from calf brain has been obtained by molecular sieve chromatography on Sephadex G-150. A high molecular weight form, EF 1A, interacts with GTP to form an EF 1A-GTP complex. GDP also reacts with EF 1, but unlike the reaction with GTP, an EF 1B-GDP complex is formed that contains a lower molecular weight and labile species of EF 1. The results also indicate that EF 1A-GTP reacts with aminoacyl-tRNA to form an aminoacyl-tRNA-EF 1B-GTP complex. These results are discussed with regard to the role of EF 1 in aminoacyl-tRNA binding to ribosomes.

Keywords: protein synthesis, aminoacyl-tRNA binding, calf brain, Sephadex G-150

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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