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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 May;69(5):1273–1276. doi: 10.1073/pnas.69.5.1273

Photoreduction of NADP+ Sensitized by Synthetic Pigment Systems

E Greenbaum 1,2, R H Austin 1,2, H Frauenfelder 1,2, I C Gunsalus 1,2
PMCID: PMC426680  PMID: 4402537

Abstract

Two synthetic pigment systems capable of enzymatically photoreducing NADP+ are described. One system contains proflavine; the other, acridine. The complete system consists of ethylene diamine tetraacetic acid (EDTA), proflavine (or acridine), ferredoxin-NADP reductase (EC 1.6.99.4), and NADP+. The two pigments initiate the photoreduction of NADP+ in different portions of the electromagnetic spectrum. Proflavine photosensitizes in the visible portion; acridine, in the ultraviolet. Neither proflavine nor acridine is structurally related to chlorophyll. The acridine system has the attractive property that the enzyme, ferredoxin-NADP reductase, is the only component of the system that absorbs appreciably in the visible region of the spectrum.

Keywords: acridine, proflavine, ferredoxin-NADP reductase, ethylene diamine tetraacetic acid

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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