Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jun;69(6):1358–1362. doi: 10.1073/pnas.69.6.1358

Light-Induced Fluorescence Changes in Chlorella, and the Primary Photoreactions for the Production of Oxygen

David Mauzerall 1
PMCID: PMC426701  PMID: 4504343

Abstract

The light-induced increases of the effective fluorescence yield in Chlorella are too slow to be primary processes in photosynthesis. The fast transient state (risetime 25 nsec, limited to the first flash) is attributed to a priming reaction for the photosystem that makes oxygen. The slower cyclical process (risetime 3 μsec, decay time 200 μsec and 2 msec) is attributed to the dark reactions that make oxygen after photoexcitation of this system. The slower cyclical process is also distinguished by a narrower emission spectrum that peaks at a shorter wavelength than the dark adapted or fast transient state. A minimum of six different fluorescent states are required to explain the data. In addition to the usual assumption about changing quantum yield of fluorescence in these processes, the data suggest that changes in cross section of optical absorption must also be considered. The slowest relaxation times observed (0.2-2 msec) are well correlated with the slow steps detected in evolution of oxygen.

Keywords: photosynthesis, flash, gated systems

Full text

PDF
1358

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTLER W. L. Effects of red and far-red light on the fluorescence yield of chlorophyll in vivo. Biochim Biophys Acta. 1962 Oct 22;64:309–317. doi: 10.1016/0006-3002(62)90739-4. [DOI] [PubMed] [Google Scholar]
  2. Bonaventura C., Myers J. Fluorescence and oxygen evolution from Chlorella pyrenoidosa. Biochim Biophys Acta. 1969;189(3):366–383. doi: 10.1016/0005-2728(69)90168-6. [DOI] [PubMed] [Google Scholar]
  3. Borisov A. Y., Godik V. I. Fluorescence lifetime of bacteriochlorophyll and reaction center photooxidation in a photosynthetic bacterium. Biochim Biophys Acta. 1970 Dec 8;223(2):441–443. doi: 10.1016/0005-2728(70)90204-5. [DOI] [PubMed] [Google Scholar]
  4. Cheniae G. M., Martin I. F. Photoactivation of the manganese catalyst of O 2 evolution. I. Biochemical and kinetic aspects. Biochim Biophys Acta. 1971 Nov 2;253(1):167–181. doi: 10.1016/0005-2728(71)90242-8. [DOI] [PubMed] [Google Scholar]
  5. Delosme R. Etude de l'induction de fluorescence des algues vertes et des chloroplastes au début d'une illumination intense. Biochim Biophys Acta. 1967 Jul 5;143(1):108–128. doi: 10.1016/0005-2728(67)90115-6. [DOI] [PubMed] [Google Scholar]
  6. Diner B. A., Mauzerall D. C. 3-(3,4-dichlorophenyl)-1, 1-dimethylurea-insensitive oxygen production in a cell-free preparation from Phormidium luridum that shows redox potential dependent coupling to one-electron oxidants. Biochim Biophys Acta. 1971 Mar 2;226(2):492–497. doi: 10.1016/0005-2728(71)90119-8. [DOI] [PubMed] [Google Scholar]
  7. Döring G., Renger G., Vater J., Witt H. T. Properties of the photoactive chlorophyll-aII in photosynthesis. Z Naturforsch B. 1969 Sep;24(9):1139–1143. doi: 10.1515/znb-1969-0911. [DOI] [PubMed] [Google Scholar]
  8. Forbush B., Kok B. Reaction between primary and secondary electron acceptors of photosystem II of photosynthesis. Biochim Biophys Acta. 1968 Aug 20;162(2):243–253. doi: 10.1016/0005-2728(68)90106-0. [DOI] [PubMed] [Google Scholar]
  9. Herron H. A., Mauzerall D. M. The light saturation curve of photosynthesis. Biochim Biophys Acta. 1970;205(2):312–314. doi: 10.1016/0005-2728(70)90262-8. [DOI] [PubMed] [Google Scholar]
  10. JOLIOT A., JOLIOT P. ETUDE CIN'ETIQUE DE LA R'EACTION PHOTOCHIMIQUE LIB'ERANT L'OXYG'ENE AU COURS DE LA PHOTOSYNTH'ESE. C R Hebd Seances Acad Sci. 1964 May 4;258:4622–4625. [PubMed] [Google Scholar]
  11. Jones L. W. Two quantum-hit requirement for delayed light emission from photosynthetic green algae. Proc Natl Acad Sci U S A. 1967 Jul;58(1):75–80. doi: 10.1073/pnas.58.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Latimer P., Bannister T. T., Rabinowitch E. Quantum Yields of Fluorescence of Plant Pigments. Science. 1956 Sep 28;124(3222):585–586. doi: 10.1126/science.124.3222.585. [DOI] [PubMed] [Google Scholar]
  13. Malkin S., Kok B. Fluorescence induction studies in isolated chloroplasts. I. Number of components involved in the reaction and quantum yields. Biochim Biophys Acta. 1966 Nov 8;126(3):413–432. doi: 10.1016/0926-6585(66)90001-x. [DOI] [PubMed] [Google Scholar]
  14. Müller A., Lumry R. The relation between prompt and delayed emission in photosynthesis. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1479–1485. doi: 10.1073/pnas.54.6.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Müller A., Lumry R., Walker M. S. Light-intensity dependence of the in vivo fluorescence lifetime of chlorophyll. Photochem Photobiol. 1969 Feb;9(2):113–126. doi: 10.1111/j.1751-1097.1969.tb05916.x. [DOI] [PubMed] [Google Scholar]
  16. Stiehl H. H., Witt H. T. Quantitative treatment of the function of plastoquinone in phostosynthesis. Z Naturforsch B. 1969 Dec;24(12):1588–1598. doi: 10.1515/znb-1969-1219. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES