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. 1972 Jun;69(6):1425–1428. doi: 10.1073/pnas.69.6.1425

Messenger RNA for Globin in the Postribosomal Supernatant of Rabbit Reticulocytes

M Jacobs-Lorena 1, C Baglioni 1
PMCID: PMC426717  PMID: 4504354

Abstract

10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for α globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total α chain mRNA is present in the postribosomal supernatant, whereas β chain mRNA is almost exclusively associated with polyribosomes.

Keywords: mouse ascites tumor extract, α chain mRNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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