Abstract
An L-cell line adapted to grow in protein-free medium is a source of material that stimulates the proliferation of serum-dependent BHK cells under otherwise protein-free conditions. The material produced by the L-cells is resolvable into separate DNA-synthetic and mitotic stimulatory activities. The stimulation of mitosis is dependent upon the presence of one or more heat-labile macromolecules, which can be concentrated by ultrafiltration, and at least one dialyzable component is essential for the initiation of DNA synthesis.
Keywords: L cells, protein-free medium
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Selected References
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- ALFRED L. J., PUMPER R. W. Biological synthesis of a growth factor for mammalian cells in tissue culture. Proc Soc Exp Biol Med. 1960 Apr;103:688–691. doi: 10.3181/00379727-103-25636. [DOI] [PubMed] [Google Scholar]
- Amborski R. L., Moskowitz M. The effects of low molecular weight materials derived from animal tissues on the growth of animal cells in vitro. Exp Cell Res. 1968 Oct;53(1):117–128. doi: 10.1016/0014-4827(68)90358-3. [DOI] [PubMed] [Google Scholar]
- Amos H. Evidence for a "governor" on protein synthesis in animal cells: positive signals in serum. Natl Cancer Inst Monogr. 1967 Sep;26:23–48. [PubMed] [Google Scholar]
- Austin P. E., McCulloch E. A., Till J. E. Characterization of the factor in L-cell conditioned medium capable of stimulating colony formation by mouse marrow cells in culture. J Cell Physiol. 1971 Apr;77(2):121–134. doi: 10.1002/jcp.1040770202. [DOI] [PubMed] [Google Scholar]
- Bachrach U. Metabolism and function of spermine and related polyamines. Annu Rev Microbiol. 1970;24:109–134. doi: 10.1146/annurev.mi.24.100170.000545. [DOI] [PubMed] [Google Scholar]
- Baserga R. Biochemical events in the cell cycle. Natl Cancer Inst Monogr. 1969 May;30:1–14. [PubMed] [Google Scholar]
- Bürk R. R. One-step growth cycle for BHK21-13 hamster fibroblasts. Exp Cell Res. 1970 Dec;63(2):309–316. doi: 10.1016/0014-4827(70)90218-1. [DOI] [PubMed] [Google Scholar]
- Clarke G. D., Stoker M. G., Ludlow A., Thornton M. Requirement of serum for DNA synthesis in BHK 21 cells: effects of density, suspension and virus transformation. Nature. 1970 Aug 22;227(5260):798–801. doi: 10.1038/227798a0. [DOI] [PubMed] [Google Scholar]
- Frankfurt O. S. Effect of hydrocortisone, adrenalin and actinomycin D on transition of cells to the DNA synthesis phase. Exp Cell Res. 1968 Sep;52(1):222–232. doi: 10.1016/0014-4827(68)90561-2. [DOI] [PubMed] [Google Scholar]
- Ham R. G. Putrescine and related amines as growth factors for a mammalian cell line. Biochem Biophys Res Commun. 1964;14:34–38. doi: 10.1016/0006-291x(63)90206-7. [DOI] [PubMed] [Google Scholar]
- Healy G. M., Parker R. C. Cultivation of mammalian cells in defined media with protein and nonprotein supplements. J Cell Biol. 1966 Sep;30(3):539–553. doi: 10.1083/jcb.30.3.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hershko A., Mamont P., Shields R., Tomkins G. M. "Pleiotypic response". Nat New Biol. 1971 Aug;232(33):206–211. [PubMed] [Google Scholar]
- Holmes R. Preparation from human serum of an alpha-one protein which induces the immediate growth of unadapted cells in vitro. J Cell Biol. 1967 Feb;32(2):297–308. doi: 10.1083/jcb.32.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kay John E., Cooke Anne. Ornithine decarboxylase and ribosomal RNA synthesis during the stimulation of lymphocytes by phytohaemagglutinin. FEBS Lett. 1971 Jul 15;16(1):9–12. doi: 10.1016/0014-5793(71)80671-3. [DOI] [PubMed] [Google Scholar]
- Mauel J., Defendi V. Regulation of DNA synthesis in mouse macrophages. II. Studies on mechanisms of action of the macrophage growth factor. Exp Cell Res. 1971 Apr;65(2):377–385. doi: 10.1016/0014-4827(71)90016-4. [DOI] [PubMed] [Google Scholar]
- Moolten F. L., Bucher N. L. Regeneration of rat liver: transfer of humoral agent by cross circulation. Science. 1967 Oct 13;158(3798):272–274. doi: 10.1126/science.158.3798.272. [DOI] [PubMed] [Google Scholar]
- Olmsted C. A. A physico-chemical study of fetal calf sera used as tissue culture nutrient correlated with biological tests for toxicity. Exp Cell Res. 1967 Nov;48(2):283–299. doi: 10.1016/0014-4827(67)90354-0. [DOI] [PubMed] [Google Scholar]
- Paul D., Lipton A., Klinger I. Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts. Proc Natl Acad Sci U S A. 1971 Mar;68(3):645–652. doi: 10.1073/pnas.68.3.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pohjanpelto P., Raina A. Identification of a growth factor produced by human fibroblasts in vitro as putrescine. Nat New Biol. 1972 Feb 23;235(60):247–249. doi: 10.1038/newbio235247a0. [DOI] [PubMed] [Google Scholar]
- Prescott D. M. Regulation of cell reproduction. Cancer Res. 1968 Sep;28(9):1815–1820. [PubMed] [Google Scholar]
- Rubin H. A substance in conditioned medium which enhances the growth of small numbers of chick embryo cells. Exp Cell Res. 1966 Jan;41(1):138–148. doi: 10.1016/0014-4827(66)90554-4. [DOI] [PubMed] [Google Scholar]
- Sakai A. Humoral factor triggering DNA synthesis after partial hepatectomy in the rat. Nature. 1970 Dec 19;228(5277):1186–1187. doi: 10.1038/2281186a0. [DOI] [PubMed] [Google Scholar]
- Simnett J. D., Morley A. R. Factors controlling growth of prostatic epithelium. A comparison of mitotic activity in mice of different ages in vivo and in organ culture. Exp Cell Res. 1967 Apr;46(1):29–36. doi: 10.1016/0014-4827(67)90406-5. [DOI] [PubMed] [Google Scholar]
- Stoker M. G., Clarke G. D., Thornton M. Density dependent stimulation of thymidine incorporation in BHK21 cells by active material released from the same cells. J Cell Physiol. 1971 Dec;78(3):345–354. doi: 10.1002/jcp.1040780304. [DOI] [PubMed] [Google Scholar]
- Thompson L. R., McCarthy B. J. Stimulation of nuclear DNA and RNA synthesis by cytoplasmic extracts in vitro. Biochem Biophys Res Commun. 1968 Jan 25;30(2):166–172. doi: 10.1016/0006-291x(68)90465-8. [DOI] [PubMed] [Google Scholar]
- Waymouth C. Somatic cells in vitro: their relationship to progenitive cells and to artificial milieux. Natl Cancer Inst Monogr. 1967 Sep;26:1–21. [PubMed] [Google Scholar]