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. 1972 Jun;69(6):1486–1489. doi: 10.1073/pnas.69.6.1486

Temperature-Dependent Intracellular Distribution of Thyroxine in Amphibian Liver

Michael D Griswold 1, Mark S Fischer 1, Philip P Cohen 1
PMCID: PMC426732  PMID: 4537638

Abstract

Rana catesbeiana tadpoles were injected with [14C]thyroxine, and the subcellular distribution of the labeled hormone was determined. At 25° the amount of isotope found in the liver was maximal after 1-2 hr and then rapidly decreased to a relatively constant value. A large percentage of the hormone was found associated with the purified nuclei isolated 24 hr after injection of [14C]thyroxine. Injection of [14C]thyroxine into tadpoles maintained at 5° resulted in a much slower but constant accumulation of isotope in the liver, with virtually no movement of thyroxine into the cell nucleus. Thyroxine was bound very tightly to the chromatin fraction of the nucleus, but extraction and chromatography revealed no chemical modification of the thyroxine itself. These results suggest the presence of two temperature-dependent processes: one concerned with the transport of thyroxine into the liver cell and a second concerned with the transport of the intracellular thyroxine into the cell nucleus. It is proposed that the latter process is involved in the low-temperature inhibition of thyroxine-induced metamorphosis.

Keywords: isotope injection, cell fractionation, accumulation in nuclei, thyroxine transport, chromatin-bound

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baxter J. D., Tomkins G. M. Specific cytoplasmic glucocorticoid hormone receptors in hepatoma tissue culture cells. Proc Natl Acad Sci U S A. 1971 May;68(5):932–937. doi: 10.1073/pnas.68.5.932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen P. P. Biochemical aspects of metamorphosis: transition from ammonotelism to ureotelism. Harvey Lect. 1966;60:119–154. [PubMed] [Google Scholar]
  3. Cohen P. P. Biochemical differentiation during amphibian metamorphosis. Science. 1970 May 1;168(3931):533–543. doi: 10.1126/science.168.3931.533. [DOI] [PubMed] [Google Scholar]
  4. Dratman M. B., Richter M. E., Lynch H. A. Incorporation of thyroxin carbon in protein fractions of Rana catesbiana tadpole nervous system, liver and tail. Endocrinology. 1970 Feb;86(2):217–224. doi: 10.1210/endo-86-2-217. [DOI] [PubMed] [Google Scholar]
  5. GORSKI J. EARLY ESTROGEN EFFECTS ON THE ACTIVITY OF UTERINE RIBONUCLEIC ACID POLYMERASE. J Biol Chem. 1964 Mar;239:889–892. [PubMed] [Google Scholar]
  6. Gorski J., Shyamala G., Toft D. Interrelationships of nuclear and cytoplasmic estrogen receptors. Curr Top Dev Biol. 1969;4:149–167. doi: 10.1016/s0070-2153(08)60483-4. [DOI] [PubMed] [Google Scholar]
  7. Gorski J., Toft D., Shyamala G., Smith D., Notides A. Hormone receptors: studies on the interaction of estrogen with the uterus. Recent Prog Horm Res. 1968;24:45–80. doi: 10.1016/b978-1-4831-9827-9.50008-3. [DOI] [PubMed] [Google Scholar]
  8. Griswold M. D., Cohen P. P. Alteration of deoxyribonucleic acid-dependent ribonucleic acid polymerase activities in amphibian liver nuclei during thyroxine-induced metamorphosis. J Biol Chem. 1972 Jan 25;247(2):353–359. [PubMed] [Google Scholar]
  9. O'Malley B. W., Spelsberg T. C., Scharder W. T., Chytil F., Steggles A. W. Mechanisms of interaction of a hormone--receptor complex with the genome of a eukaryotic target cell. Nature. 1972 Jan 21;235(5334):141–144. doi: 10.1038/235141a0. [DOI] [PubMed] [Google Scholar]
  10. Tata J. R. Hormones and the synthesis and utilization of ribonucleic acids. Prog Nucleic Acid Res Mol Biol. 1966;5:191–250. doi: 10.1016/s0079-6603(08)60235-4. [DOI] [PubMed] [Google Scholar]
  11. Tata J. R. Simultaneous acquisition of metamorphic response and hormone binding in Xenopus larvae. Nature. 1970 Aug 15;227(5259):686–689. doi: 10.1038/227686a0. [DOI] [PubMed] [Google Scholar]
  12. Williams D., Gorski J. A new assessment of subcellular distribution of bound estrogen in the uterus. Biochem Biophys Res Commun. 1971 Oct 1;45(1):258–264. doi: 10.1016/0006-291x(71)90078-7. [DOI] [PubMed] [Google Scholar]
  13. Yamamoto K., Kanski D., Frieden E. The uptake and excretion of thyroxine, triiodothyronine and iodide in bullfrog tadpoles after immersion or injection at 25 degrees and 6 degrees. Gen Comp Endocrinol. 1966 Jun;6(3):312–324. doi: 10.1016/s0016-6480(66)80019-9. [DOI] [PubMed] [Google Scholar]

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