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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jun;69(6):1531–1536. doi: 10.1073/pnas.69.6.1531

A Novel DNA Polymerase Activity Found in Association with Intracisternal A-Type Particles

Samuel H Wilson 1, Edward L Kuff 1
PMCID: PMC426742  PMID: 4504367

Abstract

A DNA polymerase activity that promotes the synthesis of poly(dT) has been found in association with intracisternal A-type particles isolated from several mouse tumors. The poly(dT) synthesis activity requires a DNA or RNA primer, is optimal at high salt concentration, prefers magnesium over manganese, and is stimulated by poly(rA). No significant incorporation of dAMP, dGMP, or dCMP was detected in the presence of several RNA and DNA template-primers. The enzyme activity differs in several of its properties from the poly(rA)-directed DNA polymerase activity associated with Rauscher murine leukemia virus.

Keywords: DNA synthesis, tumors, virus-like particles, poly(rA), myeloma

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Selected References

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  1. Aaronson S. A., Parks W. P., Scolnick E. M., Todaro G. J. Antibody to the RNA-dependent DNA polymerase of mammalian C-type RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 May;68(5):920–924. doi: 10.1073/pnas.68.5.920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  4. Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DALTON A. J., POTTER M., MERWIN R. M. Some ultrastructural characteristics of a series of primary and transplanted plasma-cell tumors of the mouse. J Natl Cancer Inst. 1961 May;26:1221–1267. [PubMed] [Google Scholar]
  6. Fridlender B., Fry M., Bolden A., Weissbach A. A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes). Proc Natl Acad Sci U S A. 1972 Feb;69(2):452–455. doi: 10.1073/pnas.69.2.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fujinaga K., Parsons J. T., Beard J. W., Beard D., Green M. Mechanism of carcinogenesis by RNA tumor viruses. 3. Formation of RNA, DNA complex and duplex DNA molecules by the DNA polymerase (s) of avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1432–1439. doi: 10.1073/pnas.67.3.1432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969 Feb;3(2):126–132. doi: 10.1128/jvi.3.2.126-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kakefuda T., Roberts E., Suntzeff V. Electron microscopic study of methylcholanthrene-induced epidermal carcinogenesis in mice: mitochondrial dense bodies and intracisternal A-particles. Cancer Res. 1970 Apr;30(4):1011–1019. [PubMed] [Google Scholar]
  10. Kuff E. L., Leuders K. K., Ozer H. L., Wivel N. A. Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor). Proc Natl Acad Sci U S A. 1972 Jan;69(1):218–222. doi: 10.1073/pnas.69.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuff E. L., Wivel N. A., Lueders K. K. The extraction of intracisternal A-particles from a mouse plasma-cell tumor. Cancer Res. 1968 Oct;28(10):2137–2148. [PubMed] [Google Scholar]
  12. Lim L., Canellakis E. S. Adenine-rich polymer associated with rabbit reticulocyte messenger RNA. Nature. 1970 Aug 15;227(5259):710–712. doi: 10.1038/227710a0. [DOI] [PubMed] [Google Scholar]
  13. Maïa J. C.C., Rougeon F., Chapeville F. Chick embryo poly (rA:dT)-dependent DNA polymerase. FEBS Lett. 1971 Oct 15;18(1):130–134. doi: 10.1016/0014-5793(71)80427-1. [DOI] [PubMed] [Google Scholar]
  14. McIntimif K. R., Asofsky R. M., Potter M., Kuff E. L. Macroglobulin-Producing Plasma-Cell Tumor in Mice: Identification of a New Light Chain. Science. 1965 Oct 15;150(3694):361–363. doi: 10.1126/science.150.3694.361. [DOI] [PubMed] [Google Scholar]
  15. Nadel E., Banfield W., Burstein S., Tousimis A. J. Virus particles associated with strain 2 guinea pig leukemia (L2C/N-B). J Natl Cancer Inst. 1967 Jun;38(6):979–981. [PubMed] [Google Scholar]
  16. POTTER M., DREYER W. J., KUFF E. L., MCINTIRE K. R. HERITABLE VARIATION IN BENCE JONES PROTEIN STRUCTURE IN AN INBRED STRAIN OF MICE. J Mol Biol. 1964 Jun;8:814–822. doi: 10.1016/s0022-2836(64)80162-5. [DOI] [PubMed] [Google Scholar]
  17. Schubert D., Humphreys S., Baroni C., Cohn M. In vitro differentiation of a mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):316–323. doi: 10.1073/pnas.64.1.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Travnicek M., Watson K. Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses. Nature. 1970 Aug 8;227(5258):563–567. doi: 10.1038/227563a0. [DOI] [PubMed] [Google Scholar]
  19. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  20. Tumilowicz J. J., Cholon J. J. Intracisternal type A particles and properties of a continuous cell line originating from a gerbil fibroma. Proc Soc Exp Biol Med. 1971 Apr;136(4):1107–1110. doi: 10.3181/00379727-136-35439. [DOI] [PubMed] [Google Scholar]
  21. Weissbach A., Schlabach A., Fridlender B., Bolden A. DNA polymerases from human cells. Nat New Biol. 1971 Jun 9;231(23):167–170. doi: 10.1038/newbio231167a0. [DOI] [PubMed] [Google Scholar]
  22. Wivel N. A., Smith G. H. Distribution of intracisternal A-particles in a variety of normal and neoplastic mouse tissues. Int J Cancer. 1971 Jan 15;7(1):167–175. doi: 10.1002/ijc.2910070119. [DOI] [PubMed] [Google Scholar]
  23. Wright B. S., O'Brien P. A., Shibley G. P., Mayyasi S. A., Lasfargues J. C. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Res. 1967 Sep;27(9):1672–1677. [PubMed] [Google Scholar]

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