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. 1972 Jun;69(6):1574–1577. doi: 10.1073/pnas.69.6.1574

Separation and Translation of the mRNAs Coding for α and β Chains of Rabbit Globin

Gary F Temple *, David E Housman †
PMCID: PMC426751  PMID: 4504371

Abstract

A method is presented to separate the mRNAs coding for α and β globins of rabbit reticulocytes. 10S RNA was extracted from the light and heavy polysomes created by incubation of reticulocytes with L-O-methylthreonine, and assayed for mRNA activity in an ascites cell-free extract. Tryptic digests of the in vitro products demonstrated that the heavy polysomes yielded β globin mRNA at least 90% free of α mRNA activity, and that the light polysomes yielded α mRNA at least 70% free of β mRNA activity.

Keywords: L-O-methylthreonine, in vitro protein synthesis, tryptic peptides

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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